The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region

Yusof, Faridah (2022) The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region. iium engineering journal. (In Press)

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Abstract

The growth conditions of bacteria producing cold-active protease isolated from the Antarctic sample were screened using one-factor-at-time (OFAT). Then, crude protease of the strain was extracted during late logarithmic phase for enzymatic assay. A strain that showed the highest enzyme activity was selected for optimization via Response Surface Method (RSM). The parameters studied were incubation temperature (4 - 36 °C), pH media (4 – 10) and NaCl concentration (0 - 8 %). Based on the OFAT results, all eight strains showed the highest growth rate at 20 °C, pH 7 and 4% (w/v) NaCl. The assay showed that the crude enzyme extracted from strain SC8 exhibited significantly higher activity (0.20 U and 0.37 U) than the positive control (0.11 U and 0.31 U) at -20 °C and 20 °C. RSM suggested that the optimized setting for growth of SC8 were at 20.5 °C, pH 6.83 and 2.05% (w/v) of NaCl with the results of the bacterial growth rate value was 3.70 ± 0.06 x 106 cells/hr. Optimal growth conditions of SC8 from this study are useful for the large-scale production of cold-active protease in future.

Item Type:	Article (Journal)
Subjects:	T Technology > T Technology (General) T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	DR FARIDAH YUSOF
Date Deposited:	01 Feb 2023 17:33
Last Modified:	01 Feb 2023 17:33
URI:	http://irep.iium.edu.my/id/eprint/102082

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