Analysis of apoptosis-inducing effect of free fucoxanthin and fucoxanthin-loaded PLGA microsphere on human lung cancer H1299 cell lines

Noviendri, Dedi and Jaswir, Irwandi and Bakhtiar, M. Taher and Mohamed, Farahidah (2024) Analysis of apoptosis-inducing effect of free fucoxanthin and fucoxanthin-loaded PLGA microsphere on human lung cancer H1299 cell lines. International Journal of Agriculture & Biology (IJAB), 31 (3). pp. 174-182. ISSN 1560–8530 E-ISSN 1814–9596

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Official URL: https://www.fspublishers.org/paper/42051

Abstract

Fucoxanthin (FX) has anticancer activity in many different human cell lines. However, this compound was sensitive to light, temperature, poor solubility in water, and low bioavailability. One way to protect FX from rapid degradation was to encapsulate it with PLGA. This study aimed to fabricate FX-loaded microspheres (FX-LMs) using PLGA and to analyze the effects of free FX and FX-LM on the induction of apoptosis in H1299 cells. The results of this study, FX-LM were successfully prepared. The particle size of FX-LM is about 9 µm, and it dissolves completely in water. Free FX showed a high cytotoxic effect on H1299 cells by giving an IC50 value of 163.40 µg/mL. However, FX-LM showed a low cytotoxic effect on H1299 cells. The concentration of FX-LM used to test the cytotoxic effect was 1482.50 µg/mL, and the IC50 value was not detected. Then, observation of the morphology using a fluorescent microscope showed that H1299 cells treated with free FX and FX-LM appeared irregularly shaped and exhibited several core features of apoptosis. After that, morphological observation by scanning electron microscope showed that H1299 cells treated with free FX and FX-LM showed shrinkage of cells, blebbing of the cell membranes, and formation of apoptosis bodies. Free FX and FX-LM induced caspase-3/7 activation on H1299 cells. Their induced apoptosis was thought to be played by cell mitochondria. In conclusion, free FX was more effective than FX-LM in cytotoxic effects and inducing apoptosis in H1299 cells. These two materials show their potential to prevent lung cancer

Item Type:	Article (Journal)
Uncontrolled Keywords:	Fucoxanthin; PLGA; Apoptosis; Lung cancer; H1299 cells
Subjects:	R Medicine > RC Internal medicine R Medicine > RC Internal medicine > RC254 Neoplasms. Tumors. Oncology (including Cancer) R Medicine > RC Internal medicine > RC254 Neoplasms. Tumors. Oncology (including Cancer) > RC261 Cancer and other malignant neoplasms R Medicine > RS Pharmacy and materia medica R Medicine > RS Pharmacy and materia medica > RS192 Materia Medica-Pharmaceutical Technology R Medicine > RS Pharmacy and materia medica > RS403 Materia Medica-Pharmaceutical Chemistry S Agriculture > S Agriculture (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART) Kulliyyah of Pharmacy > Department of Pharmaceutical Technology Kulliyyah of Pharmacy
Depositing User:	Prof. Dr. Irwandi Jaswir
Date Deposited:	12 Oct 2026 16:03
Last Update:	12 Oct 2026 16:03
Queue Number:	2026-10-Q5531
URI:	http://irep.iium.edu.my/id/eprint/131833
Indexed In:	SCOPUS, Google Scholar

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