Observational effect of Chitin and Chitosan from Horseshoe crab’s exoskeleton on the test animal, zebrafish Danio Rerio, under the laboratory condition

Wan Omar, Wan Syazzawani and Sulaiman, Wan Mohd Azizi Wan and Kassim, Zaleha and Elgharbawy, Amal A. M. (2022) Observational effect of Chitin and Chitosan from Horseshoe crab’s exoskeleton on the test animal, zebrafish Danio Rerio, under the laboratory condition. International Journal of Biochemistry & Physiology (IJBP), 7 (2). pp. 1-9. ISSN 2577-4360

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Abstract

Being a component of crustacean’s exoskeleton, chitin is one of main carbohydrate found on earth. Its derivative, chitosan can be isolated through deacetylation process of chitin. Due to their potential in pharmaceutical field, this study is using chitin and chitosan isolated from horseshoe crab to be tested on zebrafish (Danio rerio) developing embryo at various volumes. By using Danioscope, morphological defects on zebrafish embryos and larvae has been observed. We found out that both chitin and chitosan affect the zebrafish in several morphological abnormalities including dented tail, accumulation of blood, stunted development, enema and no pigmentation observed. Percentage of mortality was also determined for three different concentrations of chitin and chitosan which are at 10µl, 50µl and 100µl that been exposed to the embryo. Chitosan shows higher mortality rate compared to chitin. Both observations and data on morphological defects and mortality rate help in determining the toxicity level of chitin and chitosan on zebrafish and their effects. Our results suggested that both compounds are toxic at certain amount, hence having a high capability to be used in combating pathogens and tumour.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Chitin; Chitosan; Zebrafish; Horseshoe Crab
Subjects:	Q Science > QL Zoology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART) Kulliyyah of Science
Depositing User:	Associate Prof. Dr. Zaleha Kassim
Date Deposited:	31 Aug 2022 08:25
Last Modified:	31 Aug 2022 08:25
URI:	http://irep.iium.edu.my/id/eprint/99744

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