The evaluation of bone regeneration following socket preservation with concentrated growth factor (CGF) and poly lactic-co-glycolic acid (PLGA) scaffold in rabbits

Mohd Noh, Nur Zety and Mohamed, Nur Aliana Hidayah and Noor, Erni (2022) The evaluation of bone regeneration following socket preservation with concentrated growth factor (CGF) and poly lactic-co-glycolic acid (PLGA) scaffold in rabbits. Archives of Orofacial Sciences, 17 (Supp 1). pp. 35-50. ISSN 1823-8602 E-ISSN 2231-7163

PDF (Article) - Published Version Restricted to Repository staff only Download (1MB) | Request a copy

Abstract

Various grafting materials are utilised to facilitate regeneration. There is currently a paradigm shift towards applying poly lactic-co-glycolic acid (PLGA), which is regarded as an excellent scaffold for tissue engineering. Concentrated growth factor (CGF) has also been reported to promote wound healing. Nevertheless, the role of PLGA microspheres as a substitute for bone graft material with CGF in bone regeneration remains unclear. This study was designed to evaluate the effect of CGF with PLGA on bone formation and the expression of alkaline phosphatase (ALP) following socket preservation. PLGA microspheres were prepared using double solvent evaporation method and observed under scanning electron microscopy (SEM). A 6 mL of rabbit’s blood was collected from the marginal ear vein and centrifuged to obtain CGF. Blood was also collected for ALP assessment from 24 New Zealand White (NZW) male rabbits subjected to the first upper left premolar extraction. Sockets were filled with CGF, PLGA, CGF+PLGA or left empty and observed with microscopic computed tomography (micro-CT) at four weeks and eight weeks. The SEM image revealed a spherical shape with interconnected pores on the surface of the PLGA particles. Repeated measures ANOVA were used to evaluate the effect of time and treatment (p < 0.05) with significant differences in bone width, height, volume, volume fraction and expression of ALP was observed with CGF+PLGA. Both CGF and PLGA have the potential as the alternative grafting materials and this study could serve as an ideal benchmark for future investigations on the role of CGF+PLGA in bone regeneration enhancement.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Concentrated growth factor; poly lactic-co-glycolic acid; regeneration; socket preservation
Subjects:	R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry Kulliyyah of Dentistry > Department of Restorative Dentistry
Depositing User:	NUR ZETY MOHD NOH
Date Deposited:	05 Aug 2022 09:32
Last Modified:	05 Aug 2022 10:39
URI:	http://irep.iium.edu.my/id/eprint/99221

Actions (login required)

View Item