HPLC profile and antihyperglycemic effect of ethanol extracts of Andrographis paniculata in normal and streptozotocin-induced diabetic rats

Ahmad, Mariam and Kasmuri, Abdul Razak and Akowuah, Gabriel Akyirem and Asmawi, Mohd Zaini and Ismail, Zhari (2007) HPLC profile and antihyperglycemic effect of ethanol extracts of Andrographis paniculata in normal and streptozotocin-induced diabetic rats. Journal of Natural Medicines, 61 (4). pp. 422-429. ISSN 1861-0293 (O), 1340-3443 (P)

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Abstract

Andrographis paniculata is a Malaysian traditional herb for treatment of fever-causing diseases and diabetes. The aim of the present study was to examine the antihyperglycemic effects of different extracts (95, 50, and 20% ethanol extracts and water extracts) of A. paniculata in normal and streptozotocin-induced diabetic rats. Only the treatment with 20% ethanol extract (at 1 g/kg for 7 h, p.o.) significantly (P < 0.01) reduced the rise in blood glucose levels in glucose-loaded rats. None of the different extracts (at 1 g/kg for 7 h, p.o.) significantly (P > 0.05) altered the blood glucose levels in normal and streptozotocin- induced diabetic rats as compared to the control group. However, administration of the 50 and 95% ethanol extracts (at 1 g/kg for 14 days, p.o.) significantly (P < 0.05) reduced fasting blood glucose and insulin levels in normal and diabetic rats compared to the pre-treatment level. HPLC profiles of the extracts were developed using andrographolide (AP) as a marker. The amounts of AP in 95, 50, and 20% ethanol extracts and water extracts were 25.8, 19.4, 2.0, and 0.8 mg/g of dry weight, respectively.

Item Type:	Article (Journal)
Additional Information:	3658/9885
Uncontrolled Keywords:	Andrographis paniculata Andrographolides Diabetes HPLC Streptozotocin
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Basic Medical Sciences
Depositing User:	Dr Abdul Razak Kasmuri
Date Deposited:	12 Jul 2013 14:51
Last Modified:	12 Jul 2013 14:51
URI:	http://irep.iium.edu.my/id/eprint/9885

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