MDMA-induced BV2 microglial cell activation in vitro

Mohamad, Nasir and Mustafa, Nor Suliana and Abu Bakar, Nor Hidayah and Musa, Ramli and Mohd Adnan, Liyana Hazwani and Ahmad, Nor Zidah and Mohd Daud, Mohd Nazri and Mohamed Hashim, Zulfa Izza (2022) MDMA-induced BV2 microglial cell activation in vitro. Journal of Cellular & Molecular Anesthesia (JCMA)., 7 (3). pp. 139-146. ISSN 2538-2462 E-ISSN 2476-5120

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Abstract

Background: 3,4-Methylenedioxymethamphetamine (MDMA) is a psychostimulant drug that induces neurotoxicity. Even though several psychostimulant substances activate microglia, little is known about MDMA's effects on these cells, and evidence of MDMA-induced microglial activation is equivocal. Materials and Methods: This study employed a murine microglial cell line, BV2, to examine the effects of MDMA on the microglia morphological changes and the survival of microglia in vitro. MDMA was incorporated into the media at the time of plating, and cell number and mitochondrial dehydrogenase activity (MTT) levels were determined in vitro. The level of pro-inflammatory cytokine TNF-α was also determined. Results: Treatment of BV2 cells with MDMA resulted in morphological changes, reduced cell viability after 24h incubation with the inhibitory concentration (IC50) value of 243.6 µg/mL, and increased TNF-α level in a dose-dependent manner. Conclusion: These findings proposed that MDMA could induce BV2 microglial cell activation in vitro and suggested that it has an essential role in developing MDMA use disorder.

Item Type:	Article (Journal)
Uncontrolled Keywords:	3, 4-methylenedioxymethamphetamine, MDMA, Microglial activation, Neurotoxicity
Subjects:	R Medicine > RC Internal medicine > RC321 Neuroscience. Biological psychiatry. Neuropsychiatry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine > Department of Psychiatry Kulliyyah of Medicine
Depositing User:	Professor Ramli Musa
Date Deposited:	07 Jul 2022 12:43
Last Modified:	07 Jul 2022 12:43
URI:	http://irep.iium.edu.my/id/eprint/98717

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