Fabrication, physicochemical and rheological characterisation of a drug-therapeutic oils (doxycycline hyclate-nigella sativa-eugenol) complex emulsion stabilised by lecithin and hydroxypropyl methylcellulose intended for delivery into periodontal pocket

Mohamed, Farahidah and Hamidon, Nurhani Emira and Adina, Anugerah Budipratama and Ardini, Yunita Dewi and Mohd Shafri, Mohd. Affendi (2022) Fabrication, physicochemical and rheological characterisation of a drug-therapeutic oils (doxycycline hyclate-nigella sativa-eugenol) complex emulsion stabilised by lecithin and hydroxypropyl methylcellulose intended for delivery into periodontal pocket. Malaysian Journal of Medicine and Health Sciences, 18 (1). pp. 20-28. ISSN 1675−8544 E-ISSN 2636-9346

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Abstract

Introduction: Earlier attempts to stabilise an emulsion, intended for chronic periodontitis treatment which composed of doxycycline hyclate (DH), Nigella sativa oil (NSO), eugenol and several combinations of surfactants failed. To solve the issue, we investigated the ability of lecithin alone and its combination with hydroxypropyl methylcellulose (HPMC) to stabilise the emulsion. Method: Compatibility between DH and other ingredients was first investigated using DSC and ATR-IR. The emulsion was characterised, firstly by preparing three phases: doxycycline/preservatives with or without HPMC (varying quantities), NSO/eugenol and lecithin/surfactants as aqueous, oil and emulsifier phases, respectively. The phases were added and emulsified sequentially at 7000 rpm (10 min) with an overhead stirrer and then at 3000 rpm (15min) using a high-shear mixer. DH assay was performed using validated HPLC method. Results: All ingredients were found to be compatible with doxycycline based on DSC, ATR-IR and supported by acceptable recovery (98.2±2.2 %) of DH from the emulsion. Stable emulsions were produced with particle size of 198.6±8.2 to 279.3±10.7 nm and zeta potential of -48.2±0.4 to -64.0±3.9 mV. The emulsions showed high viscosity (~200 Pa.s) at zero shear rate and exhibited shear-thinning flow upon increased in shear stress yielding viscosity of ~3 Pa.s at 100 s-1 indicating pseudoplastic behaviour suitable for pre-filled syringe packaging intended for delivery into periodontal pocket. Conclusion: Lecithin is an excellent emulsifier that can also impart pseudoplasticity for a complex emulsion constitute of drug and natural oils. This could pave the way for a more complex emulsion formu- lation fusing contemporary and therapeutic oils

Item Type:	Article (Journal)
Additional Information:	6845/96571
Uncontrolled Keywords:	Lecithin, Doxycycline, Nigella sativa, Eugenol, Chronic periodontitis
Subjects:	R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica R Medicine > RS Pharmacy and materia medica > RS192 Materia Medica-Pharmaceutical Technology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Dentistry Kulliyyah of Dentistry > Department of Paediatric Dentistry and Dental Public Health Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User:	Dr Mohd Affendi Mohd Shafri
Date Deposited:	03 Feb 2022 14:30
Last Modified:	28 Mar 2022 08:25
URI:	http://irep.iium.edu.my/id/eprint/96571

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