Isolation and identification of wild yeast from Malaysian grapevine and evaluation of their potential antimicrobial activity against grapevine fungal pathogens

Sabaghian, Simin and Braschi, Giacomo and Vannin, Lucia and Patrignani, Francesca and Samsulrizal, Nurul Hidayah and Lanciotti, Rosalba (2021) Isolation and identification of wild yeast from Malaysian grapevine and evaluation of their potential antimicrobial activity against grapevine fungal pathogens. Microorganisms, 9 (12). pp. 1-15. E-ISSN 2076-2607

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Abstract

Pathogenic fungi belonging to the genera Botrytis, Phaeomoniella, Fusarium, Alternaria and Aspergillus are responsible for vines diseases that affect the growth, grapevine yield and organolep‐ tic quality. Among innovative strategies for in‐field plant disease control, one of the most promising is represented by biocontrol agents, including wild epiphytic yeast strains of grapevine berries. Twenty wild yeast, isolated and molecularly identified from three different Malaysian regions (Per‐ lis, Perak and Pahang), were evaluated in a preliminary screening test on agar to select isolates with inhibition against Botrytis cinerea. On the basis of the results, nine yeasts belonging to genera Han‐ seniaspora, Starmerella, Metschnikowia, Candida were selected and then tested against five grape berry pathogens: Aspergillus carbonarius, Aspergillus ochraceus, Fusarium oxysporum, Alternaria alternata and Phaeomoniella chlamydospora. Starmerella bacillaris FE08.05 and Metschnikowia pulcherrima GP8 and Hanseniaspora uvarum GM19 showed the highest effect on inhibiting mycelial growth, which ranged between 15.1 and 4.3 mm for the inhibition ring. The quantitative analysis of the volatile organic compound profiles highlighted the presence of isoamyl and phenylethyl alcohols and an overall higher presence of low‐chain fatty acids and volatile ethyl esters. The results of this study suggest that antagonist yeasts, potentially effective for the biological control of pathogenic moulds, can be found among the epiphytic microbiota associated with grape berries.

Item Type:	Article (Journal)
Additional Information:	8022/94848
Uncontrolled Keywords:	grapevines; volatile organic compounds; plant pathogens; epiphytic yeast; antifungal activity; biocontrol agents; Starmerella bacillaris; Metschnikowia pulcherrima
Subjects:	S Agriculture > S Agriculture (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science Kulliyyah of Science > Department of Plant Science
Depositing User:	Dr Nurul Hidayah Samsulrizal
Date Deposited:	16 Dec 2021 12:07
Last Modified:	29 Dec 2021 10:03
URI:	http://irep.iium.edu.my/id/eprint/94848

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