Expression of GBS virulence genes in high vaginal swabs of symptomatic pregnant women at Hospital Tengku Ampuan Afzan, Kuantan

Muhammad Faiz Matsuno, Aishah Sarah and Kamalullail, Nadhir Farhan and Muhamad, Ariff Aiman and Eddi, Khairul Ikhwan and Abdul Latif, Balqis Nur Aisyah and Supangat, Muhamad Haziq and Faiz, Fathul Aiman and Azmi, Asif Fikri and Mohd Ishak, Noor Fatin Nabilah and Saaidon, Nur Sakeenah and Hanizam, Nur Hannani and Ripin, Intan Nurfatihah and Mohd Taha, Nur Nabilah Safwah and Abdul Rahman, Ainul Subaitha and Arifin, Siti Mariah and Mustapha Rounal, Puteri Farah Diba and Mustafa Mahmoud, Mohammed Imad Al-Deen and Ismail, Hamizah and Wahid, Hanan Hamimi (2021) Expression of GBS virulence genes in high vaginal swabs of symptomatic pregnant women at Hospital Tengku Ampuan Afzan, Kuantan. In: International Undergraduate Medical Research Conference 2021, 2 - 3 November 2021, Virtual (ZOOM). (Unpublished)

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Abstract

During pregnancy, group B streptococcus (GBS) colonization is known as one of the risk factors for preterm birth and consequently neonatal infections. Previous in-vitro experiments using human cells and in vivo animal models have portrayed the important roles of these virulence factors including hemolytic pigment (CylE), hyaluronidase (HylB), serine-rich protein (Srr) and bacterial surface adhesion of GBS (BsaB) in mediating GBS colonization and intrauterine ascending infection, leading to preterm delivery. The aim of this study is to investigate the association between mRNA expression of women and preterm delivery. GBS isolates were obtained from high vaginal swabs of pregnant women(n=40) with gestational age less than 37 weeks and symptoms including preterm labour, preterm premature rupture of membrane (pPROM), vaginal discharge and vaginal bleeding. Socio-demographic details, obstetric history and delivery outcomes of these women were also enquired. RNA was extracted from these GBS isolates and RT-qPCR was performed to determine the relative mRNA expression of GBS virulence genes including CylE,HylB, Srr and BsaB. Socio-demographic details and obstetric history were not found to be associated with the delivery outcomes of these women. Women with preterm labour and pPROM who delivered prematurely were demonstrated with higher expression of HylB and Cyl Egenes, in comparison to women with term delivery. The expression of Srr and BsaB genes were both similar between symptomatic pregnant women who had term and preterm delivery. Theseresultssuggestthatfollowingvaginalcolonization,bothCylEandHylBgenespossiblycontributetointrauterineascendinginfectionandinflammation,leading to preterm delivery in humans.Thus, hemolytic pigment and hyaluronidase may be targeted for exploratory and pre-clinical stages of vaccine development, which is a good alternative to intrapartum antibiotic prophylaxis in order to prevent neonatal GBS infections.

Item Type:	Conference or Workshop Item (Slide Presentation)
Additional Information:	7933/93884
Subjects:	R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine > Department of Basic Medical Kulliyyah of Medicine > Department of Obstetric & Gynecology
Depositing User:	Mrs Norsyafini Ahmad Marzuki
Date Deposited:	24 Nov 2021 09:56
Last Modified:	24 Nov 2021 09:56
URI:	http://irep.iium.edu.my/id/eprint/93884

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