An in vitro anticancer activity evaluation of neolamarckia cadamba (Roxb.) bosser leaves’ extract and its metabolite profile

Razali, Shakirah and Khan, Al’aina Yuhainis Firus and Khatib, Alfi and Ahmed, Qamar Uddin and Wahab, Ridhwan Abdul and Zakaria, Zainul Amiruddin (2021) An in vitro anticancer activity evaluation of neolamarckia cadamba (Roxb.) bosser leaves’ extract and its metabolite profile. Frontiers in Pharmacology, 12. pp. 1-11. ISSN 1663-9812

PDF (Article) - Published Version Restricted to Registered users only Download (1MB) | Request a copy

Abstract

The leaves of Neolamarckia cadamba (NC) (Roxb.) Bosser (family: Rubiaceae) aretraditionally used to treat breast cancer in Malaysia; however, this traditional claim is yet to be scientifically verified. Hence, this study was aimed to evaluate the anticancer effect of NC leaves’ ethanol extract against breast cancer cell line (MCF-7 cells) using an in vitro cell viability, cytotoxicity, and gene expression assays followed by the gas chromatography analysis to further confirm active principles. Results revealed 0.2 mg/ml as the half maximal inhibitory concentration (IC50) against MCF-7. The extract exerted anticancer effect against MCF-7 cells in a dose- and time-dependent manner. The cell cycle assay showed that the extract arrested MCF-7 cells in the G0/G1 phase, and apoptosis was observed after 72 h by the Annexin-V assay. The gene expression assay revealed that the cell cycle arrest was associated with the downregulation of CDK2 and subsequent upregulation of p21 and cyclin E. The extract induced apoptosis via the mediation of the mitochondrial cell death pathways. A chromatography analysis revealed the contribution of D-pinitol and myo-inositol as the two major bioactive compounds to the activity observed. Overall, the study demonstrated that NC leaves’ ethanol extract exerts anticancer effect against MCF-7 human breast cancer cells through the induction of apoptosis and cell cycle arrest, thereby justifying its traditional use for the treatment of breast cancer in Malaysia.

Item Type:	Article (Journal)
Additional Information:	6915/93037
Uncontrolled Keywords:	Neolamarckia cadamba, breast cancer, gas chromatography, gene expression assay, mechanisms of action, in vitro anticancer
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. Alfi Khatib
Date Deposited:	15 Oct 2021 10:18
Last Modified:	15 Oct 2021 10:18
URI:	http://irep.iium.edu.my/id/eprint/93037

Actions (login required)

View Item