Baba, Mohd Shukri and Abu Hassan, Zainal Abidin
(2017)
In-vivo antiparasitic assessment of allicin against the growth and survival of haemoflagellate, Trypanosoma evansi.
In: Regional Conference on Materials and ASEAN Microscopy Conference 2017, 12-13 December 2017, Penang.
(Unpublished)
Abstract
Allium sativum is widely manipulated as alternative medicine and in ethnopharmacological studies. Towards the end of this study, the authors demonstrated how the manipulation of bioactive compound namely allicin, extracted from the bark of Allium sativum (garlic), promisingly can solve the endemicity of vector-borne disease called trypanosomiasis. By assessing the cell morphological changes and toxicity assessment of blood enzymes and vital organs, allicin was compared with Berenil (C18H22N8O3) on the growth and survival of the animal haemoflagellate Trypanosoma evansi. Groups of male ICR strain mice (6 – 8 weeks old, 20 – 25g body weight) were intraperitoneally (i.p) infected with the parasite at 5.0 × 103 T. evansi per mouse and orally given pre-, concurrent- and post-infection treatments with 0.1 ml of allicin at 10 µg/ml per mouse. By using Giemsa stained blood slides and examined under the light and scanning electron microscopes (SEM), there was a positive correlation (p ≤ 0.05, n = 6) between the mice survival time and the ability to inhibit the parasites growth in pre-infection treatment group. The mice in this group was also recorded the longest pre-patent (42.19 ± 1.2 days) and survival (264.58 ± 0.6 days) period. The morphological changes of T. evansi cells were observed. Besides, the results for biochemical tests were positively situated in the normal ranged level as well as no abnormalities found on the selected vital organs. This study significantly evidenced that allicin could be manipulated for the preservation and welfare of human beings, animals and environment.
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