Association of oral microbiota properties on hyaluronidase and biofilm formation

Mohd Zahir, Nur Syarafina and Abdul Halim, Nabila Huda and Ahmad Yusof @ Hanafi, Hanani (2021) Association of oral microbiota properties on hyaluronidase and biofilm formation. ASM Science Journal, 15. pp. 1-7. ISSN 1823-6782 E-ISSN 2682-8901

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Abstract

Correlation between hyaluronidase (Hyl) activity and biofilm detachment in a few bacterial species was found. However, it is unclear if this association applies to bacterial species or for more general bacterial characteristics. This study determined the association between biofilm production and Hyl activity among bacterial isolates from the oral cavity of healthy subjects, and its association with Gram staining group, colony surface morphology and bacteria shape. The swab was taken from the tongue, cheek and entire teeth surfaces of 35 subjects, and tested for biofilm through modified microtiter plate assay while Hyl production was screened through HA rapid plate method. Forty�four isolates were found, each 50% are Gram-positive, and Gram-negative bacteria, with the majority are cocci and non-mucoid colony. More than 70% of isolates are moderate and strong; (n= 17, 38.6%) and (n=15, 34.1%) respectively for biofilm production; and 68.2% are Hyl producer. A significant association was found between Hyl and bacterial shape (p=0.018) and colony morphology (p=0.018), while other association is not significantly measured, including between Hyl and biofilm (p=0.659). This study showed that biofilm production is not affected by the characteristics of the bacteria to produce or not produce hyaluronidase. Meanwhile, Hyl production is prone in rod shape and mucoid isolates which need further investigations.

Item Type:	Article (Journal)
Additional Information:	7232/92042
Uncontrolled Keywords:	biofilm; hyaluronidase; colony morphology; Gram-positive; Gram-negative; bacterial shape
Subjects:	Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Allied Health Sciences
Depositing User:	Dr Hanani Ahmad Yusof @ Hanafi
Date Deposited:	07 Sep 2021 16:51
Last Modified:	07 Sep 2021 16:51
URI:	http://irep.iium.edu.my/id/eprint/92042

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