Identification of BRCA1 tumor suppressor gene expression as a DNA repair gene in frozen cleavage embryo cell at IIUM Fertility Centre

Abdul Razak, Redzuan Nul Hakim and Abdul Wahab, Azantee Yazmie and Ramli, Roszaman and Nik Osman, Nik Mohammed Faez and Nik Hassan, Nik Fakhuruddin and Muhammad, Hussin and Mazlan, Muhammad Alif and Md Isa, Muhammad Lokman (2020) Identification of BRCA1 tumor suppressor gene expression as a DNA repair gene in frozen cleavage embryo cell at IIUM Fertility Centre. Malaysian Journal of Biochemistry and Molecular Biology, 23 (3). pp. 83-87. ISSN 1511-2616 E-ISSN 2600-9005

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Abstract

BRCA1 is known as the essential tumor suppressor gene in human that expresses a protein that called breast cancer type 1 susceptibility protein. It has two main functions which are repairing the damaged DNA and also inducing the apoptotic mechanism to eliminate the cell when mutated DNA could not be repaired. In this study, all the embryo samples that have been used were considered as grade 1 according to Embryo Grading. This study aimed to investigate BRCA1 gene expression in relation to the human embryo quality. The RNA from the cleavage embryo was extracted and was tested by Reverse-Transcriptase Polymerase Chain Reaction (rt- PCR) and gel electrophoresis. The results showed that BRCA1 gene expression was expressed in frozen cleavage embryos. Furthermore, the statistical analysis revealed that there are no significant differences among the fragmentation rates and also in Pre-embryo Genome Activation (EGA) and Embryo Genome Activation (EGA) phases. This study would be beneficial in terms of controlling the DNA repair gene activity during the pre-implantation phase. The expression of BRCA1 could be a useful health assessment indicator for embryos.

Item Type:	Article (Journal)
Additional Information:	9607/90017
Uncontrolled Keywords:	BRCA1, Frozen cleavage embryo, embryonic genome activation, fragmentation, reverse-transcriptase Polymerase Chain Reaction (rt-PCR)
Subjects:	R Medicine > R Medicine (General) R Medicine > RG Gynecology and obstetrics
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Medicine Kulliyyah of Medicine > Department of Obstetric & Gynecology Kulliyyah of Nursing Kulliyyah of Nursing > Department of Basic Medical Sciences for Nursing
Depositing User:	Redzuan Nul Hakim Abdul Razak
Date Deposited:	31 May 2021 16:00
Last Modified:	31 May 2021 16:00
URI:	http://irep.iium.edu.my/id/eprint/90017

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