Ara, Bayasrin and Urmi, Umme Laila and Haque, Tanjum Ara and Nahar, Shamsun and rumnaz, Adity and Ali, tamanna and Shah Alam, Mohammed and Md. Mosaddek, Abu Syed and A. Rahman, Nor Azlina and Haque, Mainul and Islam, Salequl (2021) Detection of mobile colistin-resistance gene variants (mcr-1 and mcr-2) in urinary tract pathogens in Bangladesh: the last resort of infectious disease management colistin efficacy is under threat. Expert Review Of Clinical Pharmacology, 14 (4). pp. 1-10. ISSN 1751-2433 E-ISSN 1751-2441
PDF (Article)
- Published Version
Restricted to Repository staff only Download (1MB) | Request a copy |
|
PDF (SCOPUS)
Restricted to Repository staff only Download (511kB) | Request a copy |
Abstract
Background: Currently, colistin-resistant pathogens emerged has become a global health concern. This study assessed the distribution of mcr-1 to mcr-5 variants with the phenotypic colistin-resistance in bacterial isolates from urinary tract infection (UTI) patients in Bangladesh. Methods: A cross-sectional study was conducted between April 2017 and March 2018 to enroll uncomplicated UTI patients, and 142 urine samples were analyzed. Uropathogens were identified using the API-20E biochemical panel and 16s rRNA gene sequencing. Polymerase chain reactions detected the mcr gene variants in the UTI isolates. The phenotypic colistin-susceptibility was determined by the Kirby–Bauer disc-diffusion method and the minimal inhibitory concentration (MIC) measurement. Results: The combined carriage of mcr-1 and mcr-2 genes in 11.4% (14/123) of urinary tract pathogens. The mcr-positive pathogens include five Escherichia coli, three Klebsiella pneumoniae, three Pseudomonas putida, two Enterobacter cloacae, and one Enterobacter hormaechei. The mcr-positive variant showed significantly higher phenotypic colistin resistance with MIC between >16 μg/mL and >128 μg/mL (p< 0.001). Over 85% of colistin-resistant isolates showed MDR phenomena. Conclusions: The emergence of the clinical MDR pathogens with resistance to a highly selective drug may lead to a lack of treatment options for the infectious diseases and spread of infection to the unaffected cohorts.
Item Type: | Article (Journal) |
---|---|
Additional Information: | 5509/89745 |
Uncontrolled Keywords: | Mobile colistin-resistance; human-mcr; urinary tract infections; Bangladesh; MDR |
Subjects: | Q Science > QR Microbiology |
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Physical Rehabilitation Sciences |
Depositing User: | Dr. Nor Azlina A Rahman |
Date Deposited: | 10 May 2021 09:58 |
Last Modified: | 30 Jun 2022 10:29 |
URI: | http://irep.iium.edu.my/id/eprint/89745 |
Actions (login required)
View Item |