Azizan, Nur Hafizah and Sharif, Faez and Zainal Abidin, Zaima Azira and Zainuddin, Zarina and Chowdhury, Ahmed Jalal Khan
(2021)
Growth optimization of mangrove Streptomyces for enhancement of
actinorhodin production.
Desalination and Water Treatment, 219.
pp. 217-222.
ISSN 1944-3994/1944-3986
Abstract
The family Streptomycetaceae, notably species in genus Streptomyces, have long been the subject of
investigation due to their well-known ability to produce secondary metabolites. Even though thousands of secondary metabolites have been discovered from Streptomyces, less attention has been
given in understanding the bioprospecting of the secondary metabolites in Streptomyces living in
mangrove ecosystem. Therefore, the current study was designed to investigate the production of
actinorhodin from mangrove Streptomyces which was grown in different parameters. Phylogenetic
analysis showed that the Streptomyces sp. K2-11 belongs to Streptomyces prasinus strain NRRL
B-12521 with 99.62% similarity. High-performance liquid chromatography analysis has detected
the presence of actinorhodin compound in Streptomyces sp. k2-11. The compound showed inhibitory activity to Bacillus subtilis and no activity against Streptococcus aureus based on disc diffusion assay. Streptomyces sp. growth in SMMS media was recorded to be the highest (1.49 × 108 cfu/
mL) compared with SYE and R2 media. The production of actinorhodin was tested on three different parameters: incubation temperature, pH and glucose levels. The highest production of
actinorhodin was recorded at 33°C (1.95 × 10–6 mol/L), pH 5 (7.1 × 10–6 mol/L) and 50% w/v glucose (9.56 × 10–6 mol/L) for 60 h of incubation. The present finding suggested that mangrove
Streptomyces sp. K2-11 produced high yield of actinorhodin under extreme or stress condition.
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