Azizan, Nur Hafizah and Zainal Abidin, Zaima Azira and Sharif, Faez
(2020)
Study of Glucose-6-Phosphate dehydrogenase activity assay in
mangrove streptomyces for actinohordin and undercylprodigiosin
production.
In:
Bioprospects of coastal ecosystem and sustainable resource management.
Noor Publication, Mauritius, pp. 17-24.
ISBN 978-620-2-79106-9
Abstract
This study evaluates the potential of using glucose-6-phosphate dehydrogenase activity assay for
Actinohordin and Undecylprodigiosin productions from mangrove Streptomyces. Previously, there were
several methods used to screen antimicrobial activities such as agar spot test and disc diffusion assay, but
those are lengthy screening methods and time consuming. Thus, to overcome the limitations plate-based
assay is suggested to enable rapid screening on secondary metabolite production of numerous samples at
one time. The development of plate-based assay was performed by optimizing glucose-6-phosphate
dehydrogenase activity assay. This coupled assay was based on the production of dihydronicotinamideadenine
dinucleotide phosphate (NADPH) whereby a right combination of nicotinamide adenine
dinucleotide phosphate (NADP) and glucose-6-phosphate (G6P) were refined. The production of NADPH
was measured at absorbance of 340 nm where reduced cofactor NADPH are absorbed readily at this
wavelength. Sample with different concentrations of crude lysate was subjected to various substrates
concentration to obtain the best activity curve. Even though elucidating clear patterns is speculative, it is
believed that some improvements or optimizations of this study could offer promising knowledge which
can serve as useful reference in future
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