Daddiouaissa, Djabir and Amid, Azura and Abdullah Sani, Muhamad Shirwan and A. M. Elnour, Ahmed
(2021)
Evaluation of metabolomics behavior of human colon cancer HT29 celllines treated with ionic liquid graviola fruit pulp extract.
Journal of Ethnopharmacology, 270.
pp. 1-8.
ISSN 0378-8741
E-ISSN 1872-7573
Abstract
Ethnopharmacological relevance: Medicinal plants have been used by indigenous people across the world for
centuries to help individuals preserve their wellbeing and cure diseases. Annona muricata L. (Graviola) which is
belonging to the Annonaceae family has been traditionally used due to its medicinal abilities including antimicrobial, anti-inflammatory, antioxidant and cancer cell growth inhibition. Graviola is claimed to be a potential
antitumor due to its selective cytotoxicity against several cancer cell lines. However, the metabolic mechanism
information underlying the anticancer activity remains limited.
Aim of the study: This study aimed to investigate the effect of ionic liquid-Graviola fruit pulp extract (IL-GPE) on
the metabolomics behavior of colon cancer (HT29) by using an untargeted GC-TOFMS-based metabolic profiling.
Materials and methods: Multivariate data analysis was used to determine the metabolic profiling, and the ingenuity pathway analysis (IPA) was used to predict the altered canonical pathways after treating the HT29 cells
with crude IL-GPE and Taxol (positive control).
Results: The principal components analysis (PCA) identified 44 metabolites with the most reliable factor loading,
and the cluster analysis (CA) separated three groups of metabolites: metabolites specific to the non-treated HT29
cells, metabolites specific to the treated HT29 cells with the crude IL-GPE and metabolites specific to Taxol
treatment. Pathway analysis of metabolomic profiles revealed an alteration of many metabolic pathways,
including amino acid metabolism, aerobic glycolysis, urea cycle and ketone bodies metabolism that contribute to
energy metabolism and cancer cell proliferation.
Conclusion: The crude IL-GPE can be one of the promising anticancer agents due to its selective inhibition of
energy metabolism and cancer cell proliferation.
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