General techniques in Western blotting: towards effective and accurate immunoblot detection of proteins from cell lysate

Mohd Nasir, Mohd Hamzah and Kamaruzzaman, Puteri Amirah Adib and Othman, Mohd Saiful and Muhamad Azdhar, Muhamad Shahrizar (2019) General techniques in Western blotting: towards effective and accurate immunoblot detection of proteins from cell lysate. [Copyright] (Unpublished)

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Abstract

Western blotting also known as immunoblotting is a technique which was widely used in the detection of proteins. The protein of interest can be derived from various biological products. Western blotting receives its name due to the popular Southern blot (for DNA detection). The specificity of this technique is mainly dictated by the set of antibodies used for each western blotting experiment. The precise binding between an antibody and its target protein epitope allows detection of highly specific amino acid sequence within a protein. Thus, proper experimental design and material selection is a must prior to the experiment.

Item Type:	Copyright
Additional Information:	5522/87553
Uncontrolled Keywords:	Western blot
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science
Depositing User:	Dr Mohd Hamzah Mohd Nasir
Date Deposited:	09 Mar 2021 13:27
Last Modified:	09 Mar 2021 13:27
URI:	http://irep.iium.edu.my/id/eprint/87553

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