Micropropagation of Labisia pumila using embryo culture

Khairuddin, Muhammad Redhuan and Azhari, Muhammad Ukasyah and Zainuddin, Zarina (2020) Micropropagation of Labisia pumila using embryo culture. Acta Scientifica Malaysia, 4 (1). pp. 1-3. ISSN 2521-5051 E-ISSN 2521-506X

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Abstract

Labisia pumila or commonly known as Kacip Fatimah is one of the plants that were used as a medicinal purpose. Previously, several studies on the regeneration of the plant through tissue culture had been conducted. L. pumila can be planted using macropropagation technique in which L. pumila can be propagated by using stem cuttings. However, the seed yields were low. This study was initiated to regenerate L. pumila using micropropagation from the embryo as the explant. Embryos were cultured on different strength MS culture mediums which are full–strength and half–strength whereby the half-strength MS medium was supplemented with 0.5 ppm benzylaminopurine (BAP) for shoot induction. Shoot formation was achieved from both media. High rate of shoot formation occurred on full-strength MS medium without plant growth regulators. Multiple shoots was established on half-strength MS medium containing 0.5 ppm BAP. Shoots elongation and plantlet establishment were produced with culture on half-strength MS medium with combination of 0.5 ppm BA and 0.5 ppm IAA.

Item Type:	Article (Journal)
Additional Information:	4409/84055
Uncontrolled Keywords:	Labisia pumila, micropropagation, full-strength MS medium, half-strength MS medium.
Subjects:	S Agriculture > SB Plant culture
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Plant Science
Depositing User:	Dr Zarina Zainuddin
Date Deposited:	02 Nov 2020 16:58
Last Modified:	12 Nov 2020 15:19
URI:	http://irep.iium.edu.my/id/eprint/84055

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