Characterisation and computational analysis of a novel lipase nanobio-based reagent for visualising latent fingerprints on water-immersed glass slides

Azman, Aida Rasyidah and Mahat, Naji Arafat and Abdul Wahab, Roswanira and Ahmad, Wan Azlina and Mohamed Huri, Mohamad Afiq and Abdul Hamid, Azzmer Azzar and Adamu, Aliyu and Mat Saat, Geshina Ayu (2020) Characterisation and computational analysis of a novel lipase nanobio-based reagent for visualising latent fingerprints on water-immersed glass slides. Process Biochemistry, 96. pp. 102-112. ISSN 1359-5113 E-ISSN 1873-3298

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Abstract

Considering the significant evidential values of fingerprints in underwater criminal investigations and the need to visualise them using a user- and environmentally-friendly reagent, development of a novel, rapid and relatively greener nanobio-based reagent (NBR) is deemed beneficial. Lipase from the commercial Candida rugosa immobilised onto acid-functionalised multi-walled carbon nanotubes (NBR) was used as the safer and cheap lipid-sensing reagent to visualise groomed whole/split fingerprints on non-porous objects immersed in stagnant tap water for up to 30 days under a laboratory-controlled setting. Attenuated Total Reflectance – Fourier Transform Spectrometry, Field Emission Scanning Electron Microscopy and bioinformatics (molecular docking and molecular dynamics simulations) were employed to characterise and confirm the attachment of NBR onto the lipid constituents of wet fingerprints. Chromatographic results further confirmed the presence of n-hexadecanoic and octadecanoic acids on fingerprints up to 30 days of immersion. Thus, NBR may potentially be useful as the future state-of-the-art fingerprint visualisation technology.

Item Type:	Article (Journal)
Additional Information:	7089/82448
Uncontrolled Keywords:	Bioinformatics, Candida rugosa lipase, Forensic science, Latent fingerprint, Nanobio-based reagent
Subjects:	Q Science > Q Science (General) R Medicine > RA Public aspects of medicine
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science Kulliyyah of Science > Department of Biotechnology
Depositing User:	AP. Dr. Azzmer Azzar Abdul Hamid
Date Deposited:	26 Aug 2020 11:37
Last Modified:	14 Sep 2020 14:43
URI:	http://irep.iium.edu.my/id/eprint/82448

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