Antibacterial activity of compounds from Azolla pinnata extracted using Soxhlet and Supercritical Fluid (SFE) methods

Mahyuddin, Husna Sabrina and Roshidi, Muhammad Ameerul Haqim and Ferdosh, Sahena and Noh, Abdul Latif (2020) Antibacterial activity of compounds from Azolla pinnata extracted using Soxhlet and Supercritical Fluid (SFE) methods. Science Heritage Journal (GWS), 4 (1). pp. 9-12. ISSN 2521-0858 E-ISSN 2521-0866

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Abstract

The propagation, extraction, and antibacterial studies of Azolla pinnata were carried out in this study. The propagation involved two fertilizers, which were chicken manure and inorganic AB fertilizer. The dry yield was extracted using two methods, which were Soxhlet and supercritical fluid extraction (SFE). Methanolic extracts were obtained and subjected to several antibacterial tests, which include the disk diffusion assay, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests, against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The results showed that AB fertilizer produced more dry yield compared to chicken manure. The extract yield from Soxhlet exhibited a higher yield than that of SFE, recording its highest at 21.20%. The findings of antibacterial tests revealed that all extracts inhibited the growth of B. subtilis and S. aureus, but none showed inhibition against E. coli and P. aeruginosa. The largest diameter of inhibition zone of 2.67 ± 1.53 mm was obtained by E2 (CM-SFE), with a MIC value of 0.125 mg/mL against B. subtilis. High MBC values further confirm that the mechanism of inhibition against B. subtilis and S. aureus were due to the bacteriostatic properties of the extracts tested.

Item Type:	Article (Journal)
Additional Information:	7165/80351
Uncontrolled Keywords:	Azolla pinnata, Soxhlet, supercritical fluid extraction, antibacterial activity.
Subjects:	Q Science > QK Botany
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science Kulliyyah of Science > Department of Plant Science
Depositing User:	Dr. Sahena Ferdosh
Date Deposited:	06 May 2020 11:11
Last Modified:	20 Jan 2021 23:35
URI:	http://irep.iium.edu.my/id/eprint/80351

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