Direct cell imprint lithography in superconductive carbon black polymer composites: process optimization, characterization and in vitro toxicity analysis

Narayanamurthy, Vigneswaran and Samsuri, Fahmi and Firus Khan, Al'aina Yuhainis and Hamzah, Hairul Aini and Baharom, Madia Baizura and Kumary, Thrikkovil Variathu and Anil Kumar, Pallickaveedu Rajanasari and Raj, Deepa K. (2020) Direct cell imprint lithography in superconductive carbon black polymer composites: process optimization, characterization and in vitro toxicity analysis. Bioinspiration and Biomimetics, 15 (1). pp. 1-15. ISSN 1748-3182 E-ISSN 1748-3190

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Abstract

Cell imprint lithography (CIL) or cell replication plays a vital role in fields like biomimetic smart culture substrates, bone tissue engineering, cell guiding, cell adhesion, tissue engineering, cell microenvironments, tissue microenvironments, cell research, drug delivery, diagnostics, therapeutics and many other applications. Herein we report a new formulation of superconductive carbon black photopolymer composite and its characterization towards a CIL process technique. In this article, we demonstrated an approach of using a carbon nanoparticle-polymer composite (CPC) for patterning cells. It is observed that a 0.3 wt % load of carbon nanoparticles (CNPs) in a carbon polymer mixture (CPM) was optimal for cell-imprint replica fabrication. The electrical resistance of the 3-CPC (0.3 wt %) was reduced by 68% when compared to N-CPC (0 wt %). This method successfully replicated the single cell with sub-organelle scale. The shape of microvesicles, grooves, pores, blebs or microvilli on the cellular surface was patterned clearly. This technique delivers a free-standing cell feature substrate. In vitro evaluation of the polymer demonstrated it as an ideal candidate for biomimetic biomaterial applications. This approach also finds its application in study based on morphology, especially for drug delivery applications and for investigations based on molecular pathways.

Item Type:	Article (Journal)
Additional Information:	5342/79824
Uncontrolled Keywords:	Direct cell imprint lithography; Superconductive carbon; Black polymer composites. :
Subjects:	N Fine Arts > NE Print media > NE2250 Lithography Q Science > QC Physics R Medicine > RE Ophthalmology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Medicine
Depositing User:	Dr Hairul Aini Hamzah
Date Deposited:	25 Jun 2020 14:02
Last Modified:	25 Jun 2020 14:02
URI:	http://irep.iium.edu.my/id/eprint/79824

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