Efficacy of bath vaccination with a live attenuated Vibrio harveyi against vibriosis in Asian seabass fingerling, Lates calcarifer

Yong, Kit Chin and Al-saari, Nurhidayu and Zulperi, Zarirah and Mohd-Aris, Aslizah and Salleh, Annas and Silvara, Santha and Mohamad, Aslah and JingYie, Lee and Saad, Mohd Zamri and Ina Salwany, Md Yasin (2020) Efficacy of bath vaccination with a live attenuated Vibrio harveyi against vibriosis in Asian seabass fingerling, Lates calcarifer. Aquaculture Research, 51 (1). pp. 389-399. ISSN 1355-557X

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Abstract

Vibrio harveyi causes vibriosis in various marine aquaculture fish species, especially when they are young. The infection subsequently leads to significant economic losses for aquaculture farms. Vaccination is recommended to control this disease. This study describes the efficacy of a live attenuated V. harveyi strain MVh_vhs (LAVh) as a vaccine candidate in controlling infection by wild‐type V. harveyi (WTVh) in Lates calcarifer. A total of 240 fingerlings were divided into four groups. Group 1 was not vaccinated and was not challenged, Group 2 was vaccinated with a formalin‐killed V. harveyi (FKVh), Group 3 was vaccinated with the LAVh before challenge and Group 4 was not vaccinated and was challenged. Bath vaccination was employed for one hour before the LAVh distribution was determined in the fish mucus, gill, liver, gut, kidney and spleen. The gills, livers, kidneys and skins were also sampled for gene expression analysis. To challenge the fish, skin abrasion was conducted before the fish were challenged by immersion with WTVh. The results revealed an extensive distribution of the LAVh in the liver and kidneys of the fish in Group 3 for the first 12 hr, resulting in mild lesions compared with Group 1. Similarly, there were significantly (p < .05) higher expressions of the Chemokine ligand 4 and major histocompatibility complex I genes in the skin and liver of the fish in Group 3 in comparison with other groups. Vaccination with LAVh resulted in a significantly high rate of survival (68%) of the fingerlings after being challenged with WTVh.

Item Type:	Article (Journal)
Additional Information:	8649/78218
Uncontrolled Keywords:	vaccination, vibriosis, Asian
Subjects:	S Agriculture > S Agriculture (General) S Agriculture > SH Aquaculture. Fisheries. Angling
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART)
Depositing User:	Br. Habidellah Ramli
Date Deposited:	31 Jan 2020 16:01
Last Modified:	06 Mar 2020 17:54
URI:	http://irep.iium.edu.my/id/eprint/78218

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