An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria

Husain, Juzaily and Griffiths, Gareth S. and Rawlinson, Andrew and Stafford, Graham and Douglas, zC.W. Ian (2019) An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria. In: Conference on BioMedical and Advanced Materials (Bio-CAM 2017), 28th-29th November 2017, Pulau Langkawi, Kedah.

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Abstract

The development of treatment strategies for periodontitis that maximise the effectiveness of antibiotics is highly desirable. Azithromycin is proving to be an effective antibiotic for treatment of refractory periodontitis which works by binding to the outer membrane of Gram-negative bacteria and subsequently inhibits protein synthesis. Lactoferrin is a membrane-active host antimicrobial protein and so the objective of this study was to determine whether the effect of azithromycin (AZM) against example periodontopathogens (Porphyromonas gingivalis and Tannerella forsythia) could be potentiated by lactoferrin. Two strains of P.gingivalis and T. forsythia were exposed to lactoferrin (LF; up to 10 mg/ml) and AZM (up to 5 μg/ml) for 0 – 72 h. The MICs for AZM were established using E-Test strips and by agar diffusion. Susceptibility to LF and LF + AZM was evaluated using diffusion assays, with and without iron depletion. The range of MIC values of AZM for P. gingivalis strains and T. forsythia was 0.16 - 0.63 μg/ml and 0.50 - 0.63 μg/ml, respectively. However, no inhibition was observed with iron saturated lactoferrin at any concentration or under iron depletion conditions nor was any effect observed on the AZM MIC by its presence. P. gingivalis and T. forsythia were inhibited by AZM but were not affected by Lf and there was no synergism between AZM and Lf.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	6296/75318
Uncontrolled Keywords:	Minimal Inhibitory Concentration; Lactoferrin; Azithromycin; Porphyromonas Gingivalis; Tannerella forsythia; Ethylenediamine dio-hydroxyphenylacetic Acid
Subjects:	R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry
Depositing User:	Dr Juzaily Husain
Date Deposited:	15 Nov 2019 08:52
Last Modified:	15 Nov 2019 08:52
URI:	http://irep.iium.edu.my/id/eprint/75318

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