Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method

Omar, Abdul Rahman and Mohd, Hair-Bejo and Ideris, Aini and Hamzah, Hairul Aini (2010) Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method. 7,659,066.

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Abstract

The present invention relates to a novel method to detect and differentiate different strains of infectious bursal disease virus (IBDV) in a chicken and other bird sample. RNA was obtained from said samples by using a pair of primer (Primer FVVC & RVVC) in a reverse transcriptase-polymerase chain reaction. Two different primer combinations (Primer IF & IVIR) and (Primer IF & RCLA) and real-time polymerase chain reaction conditions were designed and optimized for rapid differentiation of very virulent and vaccine strains of IBDV based on detection of signatory threshold cycle (Ct) and melting temperature (Tm) values.

Item Type:	Patent
Additional Information:	5342/7475
Uncontrolled Keywords:	Patent, IBDV, infections bursal disease virus, molecular biology method
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine
Depositing User:	Dr Hairul Aini Hamzah
Date Deposited:	20 Dec 2011 13:41
Last Modified:	21 Dec 2011 09:13
URI:	http://irep.iium.edu.my/id/eprint/7475

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