Inhibition of Burkholderia cenocepacia H111 quorum sensing system by environmental bacterial isolates

Wahjudi, Mariana and Kurniawan, W D and Gultom, M T and Artadana, Ida Bagus Made and Mohamad Puad, Noor Illi (2019) Inhibition of Burkholderia cenocepacia H111 quorum sensing system by environmental bacterial isolates. IOP Conference Series: Earth and Environmental Science, 293. pp. 1-9. ISSN 1755-1307 E-ISSN 1755-1315

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Abstract

Burkholderia cenocepacia H111 is an opportunistic pathogen which is difficult to eradicate due to its virulence factors, specifically biofilm. The ability of B. cenocepacia to form biofilm is regulated by quorum sensing (QS) system using a signal molecule. Environtmental bacteria often showed the ability to inhibit the QS of other species because they have special and unique metabolites or enzymes. In previous research several environtmental bacteria was isolated from few places in Java Island. However, until now the potential of these bacteria have not been investigated yet. This study was conducted with the aim to investigate the effect of these environmental bacterial isolates, i.e. Bacillus sp., Stenotrophomonas maltophilia, Enterobacter ludwigii and Kosakonia radicincitans on quorum sensing system of B. cenocepacia H111, particularly on quantities of acyl homoserine lactones (AHLs) and biofilm that were produced. The quantity of AHL molecule is measured by biosensor assay using Escherichia coli JM109 pSB401 while the quantity of biofilm formation was quantified by crystal violet method. The result showed that supernatants of all these environtmental bacteria have no impact in decreasing of AHL nor biofilm. On the other hands, all the crude extracts showed a reduction either to AHL molecule and biofilm formation. The Bacillus sp. crude extract revealed the most reducing effect, with 84 % for AHL molecule and 71 % for biofilm. It can be concluded that crude extracts of all environmental bacteria have quorum quenching activity to B. cenocepacia H111 cells, specifically in reducing AHL levels and biofilm quantity. Crude extract from Bacillus sp. gave the highest inhibitory effect.

Item Type:	Article (Journal)
Additional Information:	5462/73655
Uncontrolled Keywords:	Acyl homoserine lactones, biofilm, pSB401, quorum quenching
Subjects:	Q Science > QR Microbiology T Technology > T Technology (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering Kulliyyah of Engineering
Depositing User:	Noor Illi Mohamad Puad
Date Deposited:	15 Aug 2019 16:20
Last Modified:	11 Sep 2019 15:59
URI:	http://irep.iium.edu.my/id/eprint/73655

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