Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes

Kamarudin, Kamarul Rahim and Mohamed Rehan, Maryam and Mohamed Rehan, Aisyah (2019) Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes. Maritime Technology and Research, 1 (2). pp. 1-19. E-ISSN 2651-205X

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Abstract

Species identification of sea cucumbers that have undergone body deformation due to extensive food processing e.g. beche-de-mer is difficult especially with the copresence of cases of unlabelled or mislabelled sea cucumber-based products in the markets. Therefore, a study was done to determine the species identities of processed sea cucumbers from selected Malaysian markets using concatenated gene sequences of non-protein-coding 12S and 16S mitochondrial rRNA genes. Phylogenetic analyses based on the distance-based Neighbour Joining method, and the character-based methods i.e. the Maximum Parsimony method, Maximum Likelihood method, and the Bayesian Analysis method of 47 ingroup sequences representing 37 processed sea cucumber specimens, 6 reference samples, and 4 additional specimens suggested the presence of 3 main clusters i.e. gamat family consisting of genus Stichopus and genus Thelenota; and timun laut family comprising family Holothuriidae. A number of 3 gamat species i.e. Stichopus horrens, Stichopus vastus, and Thelenota anax were recorded. Meanwhile, the specimens of Holothuria (Halodeima) atra, Holothuria (Halodeima) edulis, Holothuria (Metriatyla) lessoni, Holothuria (Mertensiothuria) leucospilota, and Holothuria (Metriatyla) scabra were the 5 timun laut species that grouped under the family Holothuriidae. The outcomes of this study can be utilised by the enforcement agencies to monitor and overcome the issues of species substitution and product mislabelling of processed sea cucumber products in Malaysian markets.

Item Type:	Article (Journal)
Additional Information:	4469/70637
Uncontrolled Keywords:	12S rRNA gene, 16S rRNA gene, Malaysian markets processed sea cucumbers, Phylogenetic analysis
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science Kulliyyah of Science > Department of Biotechnology
Depositing User:	Mrs Aisyah Mohamed Rehan
Date Deposited:	05 Mar 2019 11:18
Last Modified:	12 Jul 2019 16:03
URI:	http://irep.iium.edu.my/id/eprint/70637

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