Antihyperglycemic activity of tinocrisposide by stimulating 3t3-L1 adipocyte cell differentiation

Adnan, Adek Zamrud and Bakhtiar, Muhammad Taher and ,, Annisa Fauzana and Afriani, Tika and Roesma, Dewi Imelda and Putra, Andani Eka (2018) Antihyperglycemic activity of tinocrisposide by stimulating 3t3-L1 adipocyte cell differentiation. Asian Journal of Pharmaceutical and Clinical Research, 11 (11). pp. 494-498. ISSN 0974-2441 E-ISSN 2455-3891

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Abstract

Objective: The aim of this study is to investigate the antihyperglycemic activity of tinocrisposide by stimulating 3T3-L1 adipocyte cell differentiation. Tinocrisposide is a furanoditerpene glycoside that was isolated from Tinospora crispa (Menispermaceae). Methods: Adipocyte cell differentiation activity of tinocrisposide in interval concentrations of 50, 25, 12.5, and 6.25 µg/ml has been investigated on 3T3-L1 cell line using insulin of 1 µg/ml as a positive and Dulbecco’s modified Eagle media (DMEM) as a negative control group. The effect of tinocrisposide was quantified with oil red O staining method by measuring an absorbance of lipid solution in isopropanol at a wavelength (λ) of 520 nm. Results: Tinocrisposide in the concentrations of 50, 25, 12.5, and 6.25 µg/ml insulin of 1 µg/ml and DMEM groups showed absorbance value of 0.7669, 0.7253, 0.6563, 0.6481, 0.954, and 0.2653, respectively. It was found that there was a significant difference statistically in lipid droplets accumulation among all groups (p<0.05) and tinocrisposide at a concentration of 50 µg/ml showed the highest lipid droplets accumulation in 3T3-L1 adipocyte cells. Conclusion: From the study, it could be concluded that tinocrisposide was able to stimulate the differentiation of adipocyte cell and had antihyperglycemic activity

Item Type:	Article (Journal)
Additional Information:	5209/70038
Uncontrolled Keywords:	3T3-L1 adipocyte cell differentiation, Antihyperglycemic, Tinocrisposide, Tinospora crispa.
Subjects:	R Medicine > RS Pharmacy and materia medica R Medicine > RS Pharmacy and materia medica > RS192 Materia Medica-Pharmaceutical Technology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User:	Dr Muhammad Taher
Date Deposited:	23 Jan 2019 17:24
Last Modified:	09 Apr 2019 11:39
URI:	http://irep.iium.edu.my/id/eprint/70038

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