Immobilisation of Candida rugosa lipase on aminated polyethylene/polypropylene microfibrous sheet modifieded with oxirane group

Akbar Tajudin, Hazimah and Al-Khatib, Ma'an Fahmi Rashid and Mirghani, Mohamed Elwathig Saeed and Mohd. Salleh, Hamzah and Nasef, Mohamed Mahmoud (2018) Immobilisation of Candida rugosa lipase on aminated polyethylene/polypropylene microfibrous sheet modifieded with oxirane group. ASM Science Journal, 11 (3). pp. 182-200. E-ISSN 1823-6782

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Abstract

An active microfibrous substrate containing aminated brush obtained by radiation-induced grafting of glycidyl methacrylate (GMA) onto a polyethylene/polypropylene (PE/PP) microfibrous sheet followed by amination reaction was prepared and used for immobilisation of Candida rugosa lipase under various conditions. The aminated microfibrous sheet was characterised by Fourier-transform infrared spectroscopy (FTIR-ATR) and field emission scanning electron microscope (FESEM). The amine group density on the aminated micro-fibrous sheet was found to be 3.33mmol/g. Response surface methodology (RSM) was applied to model and optimise the immobilisation conditions including immobilisation time (2-6 h), medium pH (pH 7-9) and enzyme/support ratio (5.0-9.0mg/cm2). The model generated from RSM was significantly correlated with the studied parameters for the residual activity of the immobilised lipase. The optimum values for immobilisation time, medium pH, and enzyme/support ratio were found to be 4.24h, pH 8, and 8.51mg/cm2 respectively. The enzymatic activity using p-nitrophenyl palmitate (pNPP) as substrate was 1.4588U/cm2 under optimum conditions. The pH endurance, storage, and thermal stability of the immobilised lipase were remarkably enhanced. The immobilised lipase can be readily recovered and more than 50% of its activity was retained following 10 cycles. The results of this study suggested that the aminated microfibrous sheet of PE/PP grafted with poly(GMA) is a promising polymer support for enzyme immobilisation with high potential for broad biocatalytic applications.

Item Type:	Article (Journal)
Additional Information:	5595/69248
Uncontrolled Keywords:	radiation induced grafting, oxirane group, enzyme immobilisation, Candida rugosa lipase, biocatalytic reactions
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART) Kulliyyah of Engineering Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr. Ma'an Alkhatib
Date Deposited:	17 Jan 2019 10:12
Last Modified:	02 May 2024 15:31
URI:	http://irep.iium.edu.my/id/eprint/69248

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