Phytochemical and pharmacological investigations of Egyptian clover, Trifolium alexandrinum L.

Ahmed, Qamar Uddin and Kaleem, Mohammed and Parveen, Nazneen and Khan, Nizam Ud Din (2006) Phytochemical and pharmacological investigations of Egyptian clover, Trifolium alexandrinum L. In: Proceedings of the International Conference on Medicinal Plants 2005, 5th-7th December 2005, Kuala Lumpur.

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Abstract

In continuation of our studies on the phytoconstituents of the Trifolium species (Fabaceae-Trifolieae), mostly occurring in India and other parts of the world, we have examined Trifolium alexandrinum L., which is also known as Barseem or Egyptian clover. Its aerial part is used as hay or pasture and seeds are used as an antidiabetic. Phytochemical data, obtained with several Trifolium species previously investigated, allowed the characterization of many secondary metabolites mainly consisting of flavonoids and aliphatic compounds structural types. The aim was to identify and characterize phenolic components in the leaves of T. alexandrinum for antimicrobial and antioxidant activities. Eight different flavonoids were detected after multistep chromatographic fractionation and preparative TLC using different solvent systems. The isolated flavonoids after purification from ethyl acetate extract were identified as 1) 5,7-dihydroxy-4'-O-methyl isoflavone, 2) 5,7,4'trihydroxy isoflavone, 3) 5,7,4'-trihydroxy flavone, 4) 5,7,3',4'-tetrahydroxy flavone, 5) apigenin 7-0-β-D-galactopyranoside, and 6) luteolin 7-0-β-glucoside by UV, IR, MS, IH-NMR, 13C-NMR analyses, direct comparison with their authentic samples and different chemical methods. Occurrence of monoflavonoids 3,4,5,6 from this plant is being reported for the first time. Further studies are still in progress to elucidate the structure of the remaining two phenolic compounds. The inhibitory potencies and capacities of different extracts against many gram positive and gram negative bacteria were also tested.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	4921/68981
Uncontrolled Keywords:	Trifolium alexandrinum, phytochemistry, Fabaceae
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr Qamar Uddin Ahmed
Date Deposited:	03 Jan 2019 08:16
Last Modified:	16 Jul 2019 09:38
URI:	http://irep.iium.edu.my/id/eprint/68981

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