Screening fungi For lipid production For consolidated bioprocessing

Hasni, Mohd Haffizi and Ahmad, Farah and Athoillah, Ahdyat Zain and Mansor, Mariatul Fadzillah (2018) Screening fungi For lipid production For consolidated bioprocessing. In: 5th International Conference Biotechnology Engineering (ICBioE ‘18), 19 - 20 September 2018, Gombak, Kuala Lumpur.

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Abstract

This paper discusses about the production of lipid and glucose by three different fungi; Aspergillus niger, Aspergillus Oryzae, and Rhizopus sp.. Biodiesel consisting of methyl esters can be produced from fungal-based lipids through transesterification. Biodiesel can be produced from various carbon substrates. However, the existing production of biodiesel from lignocellulosic biomass is not economically feasible due to the multiple processes required; (1) pre-treatment process to break down complex structure of lignin-hemicellulose-cellulose matrix, (2) saccharification of cellulose via cellulases and xylanases into simple sugars, and (3) fermentation of simple sugars. Consolidated bioprocess (CBP) combines saccharification and fermentation into one reactor. A single microorganism needs to be able to produce cellulase, break down cellulose to produce simple sugars, and ferment it to produce lipid in CBP. The objective of this study is to find the most promising fungi amongst the studied strains to be used in CBP by investigating the fungi’s ability to produce lipid. M9 medium supplemented with carboxymethylcellulose was used for glucose production and was incubated for 6 days. Basic media was used for lipid production and Bligh and Dyer lipid extraction method was used to extract lipid from fungi. The results showed that Rhizopus sp. has the potential to be promising microorganisms for CBP as it has the highest lipid productivity and lowest standard deviation of glucose production in comparison to the other fungi. Although it did not produce the highest glucose concentration, this may be solved in subsequent experiment by media optimization.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	7967/68528
Uncontrolled Keywords:	Biodiesel, Glucose, Consolidated bioprocess, Lipid, Fungi
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering T Technology > TP Chemical technology > TP248.13 Biotechnology T Technology > TP Chemical technology > TP315 Fuel T Technology > TP Chemical technology > TP500 Fermentation industries. Beverages. Alcohol T Technology > TP Chemical technology > TP670 Oils, fats, waxes
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Farah Binti Ahmad
Date Deposited:	03 Jan 2019 11:51
Last Modified:	03 Jul 2019 15:24
URI:	http://irep.iium.edu.my/id/eprint/68528

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