Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology

Ulhiza, Tami Astie and Mohamad Puad, Noor Illi and Azmi, Azlin Suhaida (2018) Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology. International Journal of Hydrogen Energy, 43 (49). pp. 22148-22158. ISSN 0360-3199

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Abstract

Sago wastewater (SWW) causes pollution to the environment due to its high organic content. Annually, about 2.5 million tons of SWW is produced in Malaysia. In this study, the potential of SWW as a substrate for biohydrogen production by Enterobacter aerogenes (E. aerogenes) was evaluated. Response Surface Methodology (RSM) was employed to find the optimum conditions. From preliminary optimization, it was found that the most significant factors were yeast extract, temperature, and inoculum size. According to Face Centered Central Composite Design (FCCCD), the maximum hydrogen concentration and yield were 630.67 μmol/L and 7.42 mmol H2/mol glucose, respectively, which is obtained from the sample supplemented with 4.8 g/L yeast extract concentration, 5% inoculum, and incubated at the temperature of 31 °C. Cumulative hydrogen production curve fitted by the modified Gompertz equation suggested that Hmax, Rmax, and λ from this study were 15.10 mL, 2.18 mL/h, and 9.84 h, respectively.

Item Type:	Article (Journal)
Additional Information:	5462/67867
Uncontrolled Keywords:	Biohydrogen; Sago wastewater; Enterobacter aerogenes; Dark fermentation Response surface methodology (RSM)
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Noor Illi Mohamad Puad
Date Deposited:	27 Nov 2018 09:33
Last Modified:	27 Jan 2019 14:46
URI:	http://irep.iium.edu.my/id/eprint/67867

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