Zoheir, Khairy MA and Abd-Rabou, Ahmed A and Harisa, Gamaleldin I. and Ashour, Abdelkader Elbadawy Abbas and Ahmad, Sheikh Fayaz and Attia, Sabry M and Bakheet, Saleh A and Abdel-Hamied, Hala E. and Abd-Allah, Adel R. A. and Kumar, Ashok (2015) Gene expression of IQGAPs and Ras families in an experimental mouse model for hepatocellular carcinoma: a mechanistic study of cancer progression. International Journal of Clinical and Experimental Pathology, 8 (8). pp. 8821-8831. ISSN 1936-2625
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Abstract
IQGAPs genes play critical role in either induction or suppression of cancer and its progression, however the relationship between Ras genes and these genes are still unclear. In this study, we tried to understand the mechanistic action of IQGAPs genes and its correlation with Ras genes in mouse hepatic cancer model. The genetic expressions of IQGAP1, IQGAP2, IQGAP3, Hras, Kras, Nras, Mras, Caspase3, and BAX were followed in both hepatocellular carcinoma and normal liver cells of Balbc mice. Genotoxic agent diethylnitrosamine (DEN)-induced hepatic cancer model was induced in male mice and recorded the occurrence of hepatocellular carcinoma by morphological and histological changes in the liver. It was observed that mRNA expressions of IQGAP1, Hras, Kras, Nras, Mras, Caspase3, and BAX genes were highly elevated in hepatocellular carcinoma cells when compared with normal liver cells, additionally their expressions increased by concentrating the dose of DEN. While, the expressions of IQGAP2 and IQGAP3 were significantly decreased in hepatocellular carcinoma cells when compared with normal liver cells, as well as their expressions decreased more with increasing the dose of DEN. It was concluded from this study that IQGAP1 has a strong signaling relationship with Ras genes in induction of cancer and it is considered as a key gene for induction or suppression of the hepatocellular carcinoma.
| Item Type: | Article (Journal) |
|---|---|
| Additional Information: | 8129/65376 |
| Uncontrolled Keywords: | IQGAPs, IQGAP1, ras, DEN, hepatocellular carcinoma |
| Subjects: | R Medicine > RM Therapeutics. Pharmacology > RM300 Drugs and their action |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Medicine Kulliyyah of Medicine > Department of Basic Medical |
| Depositing User: | Dr. Abdelkader Ashour |
| Date Deposited: | 14 Sep 2018 17:21 |
| Last Modified: | 14 Sep 2018 17:21 |
| URI: | http://irep.iium.edu.my/id/eprint/65376 |
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