Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kb ligand (RANKL) in subchondral bone during the development of osteoarthritis

Akman, Akma Azmiera and Talib, Ain Aisyah and Md Yusof, Nur Azirah and Khalid, Kamarul Ariffin and Yusof, Nurul Asyiqin and Abdul Ghani, Radiah and Hamdan, Asmah Hanim and Zamli, Zaitunnatakhin (2018) Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kb ligand (RANKL) in subchondral bone during the development of osteoarthritis. In: The 15th Symposium of the Malaysian Society of Applied Biology (MSAB 2018), 29th June - 1st July 2018, Melaka. (Unpublished)

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Abstract

Osteoarthritis (OA) is a degenerative joint disease that is characterized by subchondral bone changes. Previous studies of OA have shown that structural changes were differed between subchondral bone plate (Sbp) and trabecular bone (Tb). However, the expression of OPG (bone formation) and RANKL (bone resorption) markers that underlying the differences between these two regions has yet to be determined. Hence, the present study aims to determine structural changes and expressions of OPG/RANKL in the Sbp and Tb during development of OA in Dunkin Hartley guinea pigs. Eighteen tibia was scanned using a micro-computed tomography (micro-CT) at three difference tome points; 10, 20 and 30 weeks of age to determine their Sbp and Tb thickness. Immunohistochemistry and histopathology methods were conducted to determine the expression of OPG/RANKL and microscopic OA score, respectively. The results showed that Sbp and Tb thickness were greater in the medial than the lateral side of tibial plateau, and increased significantly with ageing (p ≤ 0.01). In addition, across the time points, the OPG/RANKL ratio of medial Sbp was initially decreased, before being increased at the final time point. In contrast, the OPG/RANKL ratio of medial Tb was initially constant but then decreased at 30 weeks of age. Interestingly, a strong correlation was observed between the Sbp thickness and OPG/RANKL ratio with microscopic OA scores (r = 0.5, p ≤ 0.05), suggesting that the Sbp plays an important role in the pathogenesis of OA.

Item Type:	Conference or Workshop Item (Speech/Talk)
Additional Information:	2295/64806
Uncontrolled Keywords:	Osteoarthritis, osteoprotegerin, receptor activator of nuclear factor kb ligand, subchondral bone plate, trabecular bone
Subjects:	R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Medicine Kulliyyah of Medicine > Department of Basic Medical Kulliyyah of Medicine > Department of Department of Orthopaedics, Traumatology & Rehabilitation Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Basic Medical Sciences
Depositing User:	Zaitunnatakhin Zamli
Date Deposited:	27 Jul 2018 09:38
Last Modified:	14 Mar 2019 10:31
URI:	http://irep.iium.edu.my/id/eprint/64806

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