Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite

Yusan, Sabriye and M. Rahman, Mokhlesur and Mohamad, Nasir A.N. and ,, Tengku M. Arrif and Abdul Latif, Ahmad Zubaidi and Md Aris, Mohd Aznan and Wan Nik, Wan Sani (2018) Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite. Journal of Analytical Methods in Chemistry, 2018. pp. 1-8. ISSN 2090-8865 E-ISSN 2090-8873

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Abstract

A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employed to probe the catalytic behavior of the biosensor. A linear calibrationplotisobtainedoverawideconcentrationrangeofglucosefrom1×10−5 to2×10−3M.Underoptimalconditions,the biosensor exhibits high sensitivity (0.34µA·mM−1), low detection limit (1×10−4M), high affinity to glucose (Km�0.02mM), and also good reproducibility (R.S.D. 2.8%, n � 10) and a stability of about ten days when stored dry at +4°C. Besides, the effectsofpHvalue,scanrate,mediatoreffectsontheglucosecurrent,andelectroactiveinterferenceofthebiosensorwerealso discussed. As a result, the biosensor exhibited an excellent electrocatalytic response to glucose as well as unique stability and reproducibility.

Item Type:	Article (Journal)
Additional Information:	4632/63788
Uncontrolled Keywords:	Amperometric; Glucose biosensor; Glucose Oxidase; Se-MCM-41; Mesoporous
Subjects:	R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica R Medicine > RZ Other systems of medicine
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine > Department of Family Medicine (Effective: 1st January 2011)
Depositing User:	Prof Dr Mohd Aznan Md Aris
Date Deposited:	23 May 2018 13:55
Last Modified:	30 Jul 2018 10:23
URI:	http://irep.iium.edu.my/id/eprint/63788

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