Anti-biofilm and anti-quorum sensing properties of ethanol and aqueous extracts of orthosiphon stamineus and andrographis paniculata leaves and stems

Yang Abd Talib, Farah Adiba and Hamzah, Hairul Aini and Mustafa Mahmoud, Mohammed Imad Al-Deen (2017) Anti-biofilm and anti-quorum sensing properties of ethanol and aqueous extracts of orthosiphon stamineus and andrographis paniculata leaves and stems. International Medical Journal Malaysia (IMJM), 16 (Supp. 1). ISSN 1823-4631

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Abstract

Introduction: Quorum sensing controls a wide spectrum of processes and phenotypic behaviours including biofilm formation that helps in the survival and virulence of bacteria in hosts. Thus, anti-quorum sensing is suggested to combat bacterial infections. This study aimed to evaluate the anti-biofilm forming and anti-quorum sensing activities which may contribute to the anti-bacterial effect of ethanol and aqueous extracts of Orthosiphon stamineus and Andrographis paniculata leaves and stems. Materials and method: General biofilm assay protocol with modification was used for the biofilm assay to test the effect of different concentration (2 mg/ml and 3 mg/ml) of extracts on the biofilm formation by Pseudomonas aeruginosa. Optical density of crystal violet/acetic acid solution was measured at 595 nm and the percentage of biofilm inhibition was calculated. In quorum quenching assay, visualization of reporter strain AI1-QQ.1 growth will be used for the detection of bio-molecules interfering with acyl homoserine lactone (AHL). Results: Extracts with the highest ability to inhibit biofilm formation were ethanol extract of O.stamineus stem and A.paniculata leaves with 77.84% at extracts concentration of 3 mg/ml. Aqueous extract of O.stamineus leaves showed the least potential with 13.41% inhibition. However, aqueous extract of A.paniculata showed zero inhibition at 3 mg/ml and two extracts (aqueous extract of O.stamineus and A.paniculata leaves) gave negative value at concentration of 3 mg/ml and 2 mg/ml,respectively. Quorum quenching assay has yet to be done. Conclusion: It could be concluded that most of the extracts possess anti-biofilm property.

Item Type:	Article (Meeting Abstract)
Additional Information:	5342/63739
Uncontrolled Keywords:	Quorum sensing controls, ethanol and aqueous extracts
Subjects:	Q Science > QR Microbiology Q Science > QR Microbiology > QR355 Virology R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine > Department of Basic Medical
Depositing User:	Dr Hairul Aini Hamzah
Date Deposited:	21 May 2018 15:43
Last Modified:	21 May 2018 15:45
URI:	http://irep.iium.edu.my/id/eprint/63739

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