Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres

Ansary, Rezaul H. and Rahman, Mohd. Mokhlesur and Mohamad, Nasir A.N. and Arrif, Tengku M and Abdul Latif, Ahmad Zubaidi and Katas, Haliza and Wan Nik, Wan Sani and Awang, Mohamed (2017) Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres. Polymers, 9 (10). pp. 1-17. ISSN 2073-4360

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Abstract

Double-walled microspheres based on poly(lactide-co-glycolide) (PLGA) are potential delivery systems for reducing a very high initial burst release of encapsulated protein and peptide drugs. In this study, double-walled microspheres made of glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA), and carboxyl-terminated PLGA were fabricated using a modified water-in-oil-in-oil-in-water (w1/o/o/w2) emulsion solvent evaporation technique for the controlled release of a model protein, lysozyme. Microspheres size, morphology, encapsulation efficiency, lysozyme in vitro release profiles, bioactivity, and structural integrity, were evaluated. Scanning electron microscopy (SEM) images revealed that double-walled microspheres comprising of Glu-PLGA and PLGA with a mass ratio of 1:1 have a spherical shape and smooth surfaces. A statistically significant increase in the encapsulation efficiency (82.52 ± 3.28%) was achieved when 1% (w/v) polyvinyl alcohol (PVA) and 2.5% (w/v) trehalose were incorporated in the internal and external aqueous phase, respectively, during emulsification. Double-walled microspheres prepared together with excipients (PVA and trehalose) showed a better control release of lysozyme. The released lysozyme was fully bioactive, and its structural integrity was slightly affected during microspheres fabrication and in vitro release studies. Therefore, double-walled microspheres made of Glu-PLGA and PLGA together with excipients (PVA and trehalose) provide a controlled and sustained release for lysozyme.

Item Type:	Article (Journal)
Additional Information:	5269/62789
Uncontrolled Keywords:	drug delivery; controlled release; double-walled microspheres; poly(lactide-co-glycolide); therapeutic protein
Subjects:	Q Science > QD Chemistry Q Science > QR Microbiology R Medicine > RE Ophthalmology R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr Mohd Mokhlesur Rahman
Date Deposited:	06 Apr 2018 12:26
Last Modified:	21 Jun 2018 10:34
URI:	http://irep.iium.edu.my/id/eprint/62789

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