Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica

Muhammad Alhassan, Alhassan and Ahmed, Qamar Uddin and Sarian, Murni Nazira and Khatib, Alfi (2017) Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica. In: 2nd International Anatomical and Biomedical Scientific Conference 2017, 1-2 Aug 2017, Serdang, Selangor.

	PDF (slide) Restricted to Registered users only Download (2MB) | Request a copy
	PDF Restricted to Registered users only Download (1MB) | Request a copy

Abstract

The leaves of Tetracera indica are used in traditional medicine for the treatment of diabetes mellitus. This study was aimed at investigating the phytochemical constituents present in T. indica leaves and their activity against digestive enzyme (alphaglucosidase) which may be part of the mechanism of the antidiabetic effect of the plant. Crude methanolic extract from the leaves of T. indica which demonstrate significant alpha glucosidase inhibitory activity was separated and purified by column chromatography of Silica gel, ODS and Sephadex LH-20, the structures of the isolated compounds were identified by UV, 1H-NMR, 13C-NMR and MS spectroscopic techniques. Five flavonoids were isolated from the leaves of T. indica, and these compounds were identified as follows: 5,7-dihydroxy-8-methoxyflavone (wogonin), 5,7,8-trihydroxyflavone (norwogonin), quercetin, 5- hydroxy-7-methoxyflavone (tectochrysin) and 41, 3, 5, 7-tetrahydroxyflavone (kaempferol). The isolated compounds show moderate to strong activity against alphaglucosidase enzymes. Insilco studies using molecular docking show the mode of interactions of the compounds with the active site residues and their binding energies. The results of this study reveal that alphaglucosidase inhibition is one of the primary mode of action of T.indica antidiabetic effect.

Item Type:	Conference or Workshop Item (Lecture)
Additional Information:	4921/61007
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr Qamar Uddin Ahmed
Date Deposited:	31 Dec 2017 22:44
Last Modified:	31 Dec 2017 23:16
URI:	http://irep.iium.edu.my/id/eprint/61007

Actions (login required)

View Item