Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy

Ahmad Azam, Amalina and Pariyani, Raghunath and Ismail, Intan Safinar and Ismail, Amin and Khatib, Alfi and Abas, Faridah and Khozirah, Shaar (2017) Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy. BMC Complementary and Alternative Medicine, 17 (1). pp. 1-13. ISSN 1472-6882

	PDF - Published Version Restricted to Repository staff only Download (2MB) | Request a copy
Preview	PDF (SCOPUS) - Supplemental Material Download (74kB) | Preview

Abstract

Background: Orthosiphon stamineus (OS) is a herb known in ethnomedicine for treating diabetes mellitus (DM). In this study, a H NMR based urine metabolomics tool has been used for the first time to identify the metabolic protective mechanism of OS in DM using Streptozotocin (STZ) induced experimental model in rats. Methods: Four different solvent extracts of OS, namely aqueous, ethanolic, 50% aqueous ethanolic and methanolic, at a dose of 500 mg/kg body weight (bw) were orally administered for 14 days to diabetic rats induced via intraperitoneal injection of 60 mg/kg bw STZ. NMR metabolomics approach using pattern recognition combined with multivariate statistical analysis was applied in the rat urine to study the resulted metabolic perturbations. Results: OS aqueous extract (OSAE) caused a reversal of DM comparable to that of 10 mg/kg bw glibenclamide. A total of 15 urinary metabolites, which levels changed significantly upon treatment were identified as the biomarkers of OSAE in diabetes. A systematic metabolic pathways analysis identified that OSAE contributed to the antidiabetic activity mainly through regulating the tricarboxylic acid cycle, glycolysis/gluconeogenesis, lipid and amino acid metabolism. Conclusions: The results of this study validated the ethnopharmacological use of OS in diabetes and unveiled the biochemical and metabolic mechanisms involved.

Item Type:	Article (Journal)
Additional Information:	6915/57413
Uncontrolled Keywords:	Orthosiphon Stamineus, Java tea, Metabolomics, Diabetes mellitus,H NMR spectroscopy
Subjects:	R Medicine > RC Internal medicine R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy
Depositing User:	Dr. Alfi Khatib
Date Deposited:	03 Jul 2017 15:39
Last Modified:	09 Mar 2018 15:56
URI:	http://irep.iium.edu.my/id/eprint/57413

Actions (login required)

View Item