An autonomous lab on a chip for space flight calibration of gravity-induced transcellular calcium polarization in single-cell fern spores

Park, J.H. and Salmi, Mari L. and Wan Salim, Wan Wardatul Amani and Rademacher, A.T. and Wickizer, B. and Schooley, Aaron and Benton, J.E. and Cantero, A. and Argote, P. and Ren, M. and Zhang, Min and Porterfield, David Marshall and Ricco, Antonio J. and Roux, S. J. and Rickus, Jenna L. (2017) An autonomous lab on a chip for space flight calibration of gravity-induced transcellular calcium polarization in single-cell fern spores. Lab on a chip, 17 (6). pp. 1095-1103. ISSN 1473-0197 (In Press)

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Abstract

This report describes the development of lab-on-a-chip device designed to measure changes in cellular ion gradients that are induced by changes in gravitational (g) forces. The bioCD presented here detects differential calcium ion concentrations outside of individual cells. The device includes sufficient replicates for statistical analysis of the gradients around multiple single cells and around control wells that are empty or include dead cells. In the data presented, the degree of the cellular response correlates with the magnitude of the g-force applied via rotation of the bioCD. The experiments recorded the longest continuous observation of a cellular response to hypergravity made to date, and they demonstrate the potential utility of this device for assaying the threshold of cells' g-force responses in spaceflight conditions.

Item Type:	Article (Journal)
Additional Information:	7116/56923
Uncontrolled Keywords:	Autonomous lab, flight calibration, gravity-induced transcellular, calcium polarization, single-cell.
Subjects:	T Technology > T Technology (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Wan Wardatul Amani Wan Salim
Date Deposited:	16 May 2017 11:29
Last Modified:	02 Apr 2018 14:56
URI:	http://irep.iium.edu.my/id/eprint/56923

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