Proteomics profiling of crassostrea iredalei exposed to toxic alexandrium tamiyavanichii and non-toxic alexandrium leei

Hamdan, Nurul Ashima and Mamat, Siti Noor Hasmiza and Abu Hassan, Muhamad Shafiq and Mohammad Noor, Normawaty and Muhamad Bunnori, Noraslinda (2017) Proteomics profiling of crassostrea iredalei exposed to toxic alexandrium tamiyavanichii and non-toxic alexandrium leei. In: 5th International Conference on Chemical, Agricultural, Biological and Environmental Sciences (CAFES-17), 18th-21st April 2017, Kyoto, Japan.

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Abstract

The black scar oyster, Crassostrea iredalei is part of filter feeder organism and found extremely responsive to alterations in the environment from any natural contamination such as harmful algal blooms (HABs). The commercial demand of oyster is therefore of utmost importance to monitor the presence and effect on related toxins at protein level. Hence, the current study involved the comparison in protein profiles of C. iredalei after exposed to toxic A. tamiyavanichi and non-toxic A. leei for 24-hour. This aimed is achievable using two-dimensional gel electrophoresis (2-DE) that can identify the differential abundances of proteins spots for oyster’s protein profiling. There were 12 spots detected in a pH range of 3-10 that can be predicted via cross-referring of the protein molecular weight and isoelectric points, pI in Expasy databases. These predicted proteins were involved in biological activities such as energy metabolism, cell regulation and stress response. Besides that, there were two proteins spots that have been expressed in the toxic sample and further identified using LCMS analysis. Both proteins; arginine kinase and transgelin, were highly expressed in intoxicated oyster due to the oxidative stress induced by the toxic microenvironment. The presence of A. tamiyavanichi caused toxin accumulation in oysters and might induce the disorder of energy metabolism. The regulation mechanism thus explains on the oyster stability during HAB outbreak.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	6604/56713
Uncontrolled Keywords:	Crassostrea iredalei, Alexandrium tamiyavanichi, A. leei, harmful algal blooms, protein profiling, paralytic shellfish poisoning toxin
Subjects:	S Agriculture > S Agriculture (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr. Noraslinda Muhamad Bunnori
Date Deposited:	16 May 2017 16:42
Last Modified:	15 Nov 2018 10:25
URI:	http://irep.iium.edu.my/id/eprint/56713

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