Fast and simple gas chromatographic method for simultaneous estimation of camphor, menthol and methyl salicylate in analgesic ointment: application in stability study

Md Adib, Noor Adibah and Mandal, Uttam Kumar and Mohamed, Farahidah and Chatterjee, Bappaditya (2017) Fast and simple gas chromatographic method for simultaneous estimation of camphor, menthol and methyl salicylate in analgesic ointment: application in stability study. Journal of Pharmaceutical Investigation. pp. 1-11. ISSN 2093-6214 E-ISSN 2093-6214

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Abstract

A simple, rapid and sensitive gas chromatographic (GC) method with flame ionization detector (FID) has been developed and validated for simultaneous estimation of camphor, menthol and methyl salicylate (MS). Camphor, menthol and MS were separated at about 2.753, 3.206 and 3.995 min respectively on a capillary column with helium (3.3 ml/min) as carrier gas within 11 min run time. Noninterference of any peak with the peaks of interest confirms the selectivity of method. Derived quantitation limits (QL) were 0.847, 0.684 and 6.507 μg/ml for camphor, menthol and MS respectively. The linear relationship ( R2 > 0.999) between analyte concentration vs detector response was established within a range of QL to 150% of label claim concentration for each analyte. Recovery of each analyte at 50, 100 and 150% of label claim concentration levels were obtained within 99.67–101.53% establishing high accuracy of the method. The method showed acceptable precision with low relative standard deviation or RSD (0.24–1.03%) between percent recoveries for each analyte. RSD for intermediate precision (inter day analysis, analyst variation) was less than 1%. The validated method was successfully applied for quantitative determination of camphor, menthol and MS in stability samples of an analgesic ointment produced by IKOP Sdn. Bhd., Malaysia.

Item Type:	Article (Journal)
Additional Information:	6688/56687
Uncontrolled Keywords:	Camphor, Menthol, Methyl salicylate, GC-FID, Validation
Subjects:	R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User:	Dr Bappaditya Chatterjee
Date Deposited:	02 May 2017 10:29
Last Modified:	21 Mar 2018 08:47
URI:	http://irep.iium.edu.my/id/eprint/56687

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