Implementation of definite screening design in optimization of in situ hydrolysis of EFB in cholinium acetate and locally produced cellulase combined system

Elgharbawy, Amal A. and Alam, Md Zahangir and Kabbashi, Nassereldeen Ahmed and Moniruzzaman, Muhammad and Jamal, Parveen (2017) Implementation of definite screening design in optimization of in situ hydrolysis of EFB in cholinium acetate and locally produced cellulase combined system. Waste and Biomass Valorization, 8 (3). pp. 838-850. ISSN 1877-2641 E-ISSN 1877-265X

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Abstract

Purpose Ionic liquids (ILs) have been found to be highly promising for lignocellulosic biomass pretreatment, due to their excellent abilities to dissolve biopolymers. However,they have found to inactivate enzymes. In previous studies,Cholinium-based ILs showed great compatibility with cellulases and thus were introduced for a combined-system. This study aims to find the optimum condition in order to achieve the maximum pretreatment-hydrolysis of empty fruit bunches (EFB). Methods Definitive screening design (DSD) was employed with seven factors that are thought to impact the process including pretreatment temperature, pretreatment time, hydrolysis time, enzyme loading, particle size, biomass loading, and IL/buffer ratio. DSD offered several solutions for optimization in which they were experimentally tested. Results The maximum sugar concentration (77 g/L) was obtained at 90 C, 70–116 min of treatment, 36–42 h of hydrolysis, 40–62 Unit/g cellulase loading, 34–35 %, w/v) biomass loading, 220–450 l particle size, and 10 % (v/v) IL to buffer ratio, respectively. The subsequent optimization by response surface methodology (RSM) revealed the temperature for treatment can drop to 75 C while fixing the pretreatment time at 100 min. Around 75 unit/g of cellulase and [22.0 % (w/v) of the biomass could be loaded to achieve a minimum of 70.0 ± 7.83 g/L of sugar, equivalent to 0.38 ± 0.08 g glucose/g and 0.48 ± 0.05 g total reducing sugar/g dry EFB. Conclusions The locally produced cellulase (PKC-Cel) from Trichoderma reesei exhibited promising results in the single-step process and can be used as an efficient approach to be optimized for fermentation to bioethanol production.

Item Type:	Article (Journal)
Additional Information:	4157/56454
Uncontrolled Keywords:	Optimization, Hydrolysis, Pretreatment, Stability, Lignocellulose, Compatible Int
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Nassereldeen Kabbashi
Date Deposited:	26 Apr 2017 09:30
Last Modified:	10 Aug 2017 11:39
URI:	http://irep.iium.edu.my/id/eprint/56454

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