Evaluation of antioxidant, total phenolsand flavonoids content and antimicrobial activities of Artocarpus altilis (breadfruit) of underutilized tropical fruit extracts

Abdul Wahab, Ridhwan and Jalal, Tara Kamal and Draman, Samsul and Md Isa, Muhammad Lokman and Abdull Rasad, Mohammad Syaiful Bahari and Omar, Muhammad Nor and Ibrahim, Muhammad (2014) Evaluation of antioxidant, total phenolsand flavonoids content and antimicrobial activities of Artocarpus altilis (breadfruit) of underutilized tropical fruit extracts. In: International Health Conference IIUM 2014, 3rd-4th Dec. 2014, Kuantan, Pahang. (Unpublished)

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Abstract

Artocarpusaltilis(breadfruit) pulp, peeland whole fruit were extracted with various solvents such as hexane, dichloromethane (DCM) and methanol the extracts examined for their antioxidant activity usingthe stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test IC50 was ranged from 55±0.89 to4851±0011μg/ml. In the β-carotene bleaching assay the antioxidant activity was 90.02±1.51% for the positive control (Trolox) and 88.34±1.31% for the pulp part of the fruit methanol extract. The total phenolic content of the crude extracts was determined using the Folin-Ciocalteuprocedure, methanol pulp part demonstrated the highest phenol content value of 6570.74±1.14 mg GAE/ g of dry sample. While the total flavonoid content was determined using the aluminium chloride colorimetric assay highest value of 2545.01±0.91 mg QE/ g indicated by pulp part of the fruit methanol extract. Theantimicrobial activity of the crude extracts was tested using disc diffusion method against pathogenic microorganisms: S. aureus, S. epidermidis, B. cereus, S. typhimurium, E. coli, K. pneumonia and C. albican. Methanol extract of pulp part was recorded to have the highest zone of inhibition against Gram-positive and Gram-negative bacteria. The MIC and MBC/MFC for the extracts were also determined using the microdilution method ranged from 4000-63 μg/ml against pathogenic microbes. The MBC/MFC values varied from 250 to 4000 μg/ml. A correlation between antioxidant activity assays, antimicrobial activity and phenolic content was established. The results shows that the various parts of A. altilis fruit extracts promising antioxidant activities have a potential bioactivities due to high content of phenolic compounds.

Item Type:	Conference or Workshop Item (UNSPECIFIED)
Additional Information:	5136/55736
Uncontrolled Keywords:	antioxidant, artocarpus altilis
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Allied Health Sciences > Department of Basic Health Sciences (Until June 2011) Kulliyyah of Allied Health Sciences
Depositing User:	Dr. Mohammad Syaiful Bahari Abdull Rasad
Date Deposited:	19 Apr 2017 11:21
Last Modified:	09 Jun 2022 15:38
URI:	http://irep.iium.edu.my/id/eprint/55736

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