The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bonemarrow mesenchymal stem cells for in vitro cartilage tissue engineering

Abdul Rahman, Rozlin and Mohamad Sukri, Norhamiza and Md Nazir, Noorhidayah and Ahmad Radzi, Muhammad Aa'zamuddin and Zulkifly, Ahmad Hafiz and Che Ahmad, Aminudin and Abdulahi Hashi, Abdurezak and Abdul Rahman, Suzanah and Sha'ban, Munirah (2015) The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bonemarrow mesenchymal stem cells for in vitro cartilage tissue engineering. Tissue and Cell, 47 (4). pp. 420-430. ISSN 0040-8166

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Abstract

Articular cartilage is well known for its simple uniqueness of avascular and aneural structure that has limited capacity to heal itself when injured. The use of three dimensional construct in tissue engineering holds great potential in regenerating cartilage defects. This study evaluated the in vitro cartilaginous tissue formation using rabbit’s bone marrow mesenchymal stem cells (BMSCs)-seeded onto poly(lactic-co-glycolic acid) PLGA/fibrin and PLGA scaffolds. The in vitro cartilaginous engineered constructs were evaluated by gross inspection, histology, cell proliferation, gene expression and sulphated glycosamino-glycan (sGAG) production at week 1, 2 and 3. After 3 weeks of culture, the PLGA/fibrin construct demonstrated gross features similar to the native tissue with smooth, firm and glistening appearance, superior histoarchitectural and better cartilaginous extracellular matrix compound in concert with the positive glycosaminoglycan accumulation on Alcian blue. Significantly higher cell proliferation in PLGA/fibrin construct was noted at day-7, day-14 and day-21(p < 0.05 respectively). Both constructs expressed the accumulation of collagen type II, collagen type IX, aggrecan and sox9, showed down-regulation of collagen type I as well as produced relative sGAG content with PLGA/fibrin construct exhibited better gene expression in all profiles and showed significantly higher relative sGAG content at each time point (p < 0.05). This study suggested that with optimum in vitro manipulation, PLGA/fibrin when seeded with pluripotent non-committed BMSCs has the capability to differentiate into chondrogenic lineage and may serve as a prospective construct to be developed as functional tissue engineered cartilage.

Item Type:	Article (Journal)
Additional Information:	4928/55098
Uncontrolled Keywords:	Cartilage tissue engineering, Poly(lactic-co-glycolic acid), Bone marrow mesenchymal stem cells, Fibrin' Scaffold
Subjects:	R Medicine > R Medicine (General) R Medicine > RD Surgery > RD701 Orthopedics
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Medicine Kulliyyah of Science > Department of Biotechnology
Depositing User:	Prof. Dr. Suzanah Abdul Rahman
Date Deposited:	20 Mar 2017 09:43
Last Modified:	18 Jul 2017 14:44
URI:	http://irep.iium.edu.my/id/eprint/55098

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