Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using candida cylindracea

Elgharbawy, Amal A.M. and Alam, Md Zahangir and Mohd. Salleh, Hamzah (2016) Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using candida cylindracea. ARPN Journal of Engineering and Applied Sciences, 11 (1). pp. 319-326. ISSN 1819-6608

	PDF - Published Version Restricted to Repository staff only Download (326kB) | Request a copy
	PDF (scopus) Download (372kB)

Abstract

This study aimed to identify the properties as well as the application of Candida cylindracea lipase produced from palm kernel cake (PKC) by solid-state fermentation (SSF). Characteristics of PKC-lipase have been investigated which include: thermal stability, pH stability, stability in organic solvents and metal ions solutions. The enzyme assays were conducted using colorimetric method and the activity was expressed as residual activity. It was revealed that the optimal temperature and pH were 37°C and 8.0, respectively. The enzyme was stable at temperature ranges of 25-45°C and pH 7.08.0 respectively.Zn 2+,Mg 2+ and Ca 2+ stimulated the enzyme while Fe 3+ inhibited its activity. The results showed that 1.0% of EDTA enhanced the lipase activity while incubation in SDS and Tween-80 resulted in the reduction of its activity.Moreover, the produced lipase exhibited some levels of stability at low concentrations of organic solvents (methanol,ethanol, 2-propanol, acetone, hexane and toluene). The application of the PKC-lipase showed promising results in hydrolysis whereas 50.48% and 45.98% of canola oil and olive oil were converted to free fatty acids (FFA), respectively. The knowledge obtained from this study can give an insight on understanding the PKC-lipase properties in order to be used in industrial and biotechnological applications, particularly in biodiesel production.

Item Type:	Article (Journal)
Additional Information:	4157/55040
Uncontrolled Keywords:	Candida cylindracea, palm kernel cake, enzyme stability, residual activity, hydrolysis.
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr. Md. Zahangir Alam
Date Deposited:	04 Feb 2017 09:42
Last Modified:	17 Apr 2019 10:05
URI:	http://irep.iium.edu.my/id/eprint/55040

Actions (login required)

View Item