Antibiotic susceptibility study of metal-gentamycin complexes against pseudomonas aeruginosa biofilms

Shahdan, Intan Azura and Mohd Sobri, Fatimah Zahra and Hanapi, Mohammad Faiz Hizzuan and Ahmad Yusof @ Hanafi, Hanani and How, Fiona Ni Foong (2016) Antibiotic susceptibility study of metal-gentamycin complexes against pseudomonas aeruginosa biofilms. In: Medical Research Symposium & Pacific Partnership in conjunction with Kuantan Research Day 2016, 3rd-5th August 2016, Kuantan, Pahang.

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Abstract

Inherent resistance of biofilm bacteria to conventional antibiotics is alarming because biofilms induce antibiotic resistance to an order of three or more in magnitude greater than those displayed by planktonic bacteria. Pseudomonas aeruginosa is an infectious organism that causes the hallmark of chronic infections including hospital acquired infections that leads to high morbidity and mortality. One of the reasons for the occurrence of resistance is its ability to form biofilms. In this study, the resistance of P. aeruginosa biofilms against a series of metal-antibiotics, an alternative to the conventional antibiotics, was investigated. Methods: A series of metal-antibiotic complexes derived from gentamicin was synthesized to give metal-gentamicin complexes. The metal contents of all the compounds were determined using Atomic Absorption Spectroscopy (AAS). Antibiotic susceptibility testing of the gentamicin-antibiotic complexes against the biofilms was conducted using broth microdilution assay. Results: The results showed that P. aeruginosa is susceptible against all the metal-gentamicin complexes, which include, Ni(II), Fe(II), Cu(II), Zn(II) and Co(II) complexes; all were tested at 0.25 to 1 mmol concentrations. Conclusions: The results show that all metal-gentamicin complexes have higher antimicrobial activity than gentamicin (0.25 to 1 mmol) on its own. Finally, mechanisms of P. aeruginosa biofilms resistance to these metal-antibiotics are also proposed.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	7232/54958
Uncontrolled Keywords:	Antibiotic resistance, biofilms, pseudomonas aeruginosa, metal-antibiotics complexes
Subjects:	Q Science > QD Chemistry Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User:	Dr Hanani Ahmad Yusof @ Hanafi
Date Deposited:	03 Feb 2017 12:05
Last Modified:	14 Apr 2017 14:45
URI:	http://irep.iium.edu.my/id/eprint/54958

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