Benzo(a)pyrene degradation and microbial community responses in composted soil

Zhu, Fengxiao and Storey, Sean and Mohd Ashaari, Mardiana and Clipson, Nicholas and Doyle, Evelyn (2017) Benzo(a)pyrene degradation and microbial community responses in composted soil. Environmental Science and Pollution Research, 24 (6). pp. 5404-1. ISSN 0944-1344 E-ISSN 1614-7499

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Abstract

Benzo(a)pyrene degradation was compared in soil that was either composted, incubated at a constant temperature of 22 °C, or incubated under a temperature regime typical of a composting process. After 84 days, significantly more (61%) benzo(a)pyrene was removed from composted soil compared to soils incubated at a constant temperature (29%) or at composting temperatures (46%). Molecular fingerprinting approaches indicated that in composted soils, bacterial community changes were driven by both temperature and organic amendment, while fungal community changes were primarily driven by temperature. Next-generation sequencing data revealed that the bacterial community in composted soil was dominated by Actinobacteria (order Actinomycetales), Firmicutes (class Bacilli), and Proteobacteria (classes Gammaproteobacteria and Alphaproteobacteria), regardless of whether benzo(a)pyrene was present or not. The relative abundance of unclassified Actinomycetales (Actinobacteria) was significantly higher in composted soil when degradation was occurring, indicating a potential role for these organisms in benzo(a)pyrene metabolism. This study provides baseline data for employing straw-based composting strategies for the removal of high molecular weight PAHs from soil and contributes to the knowledge of how microbial communities respond to incubation conditions and pollutant degradation.

Item Type:	Article (Journal)
Additional Information:	5512/54565
Uncontrolled Keywords:	Benzo(a)pyrene . Soil . Composting . Bioremediation . Microbial community . PAH . Amplicon sequencing
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr. Mardiana Mohd. Ashaari
Date Deposited:	16 Jan 2017 15:39
Last Modified:	09 Jul 2019 12:35
URI:	http://irep.iium.edu.my/id/eprint/54565

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