In vitro antibacterial activity of Vernonia amygdalin a leaves extracts against pathogen of heart rot disease

Abdul Aziz, Noorshilawati and Mazlan, Hamimah and Abdullah, Nor Suraya and Omar, Muhammad Nor (2016) In vitro antibacterial activity of Vernonia amygdalin a leaves extracts against pathogen of heart rot disease. In: Regional Conference on Sciences, Technology and Social Sciences (RCSTSS 2016), 4th-6th December 2016, Cameron Highland, Pahang. (Unpublished)

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Abstract

Heart rot disease is one of the major diseases in pineapple cultivation in Malaysia. Josapine and N36 are among varieties that vulnerable to the heart rot disease. The infection can reduce up to 50% yield. In vitro antibacterial activity of four Vernonia amygdalina leaves extract (hexane, chloroform, methanol and aqueous) was carried out and evaluated against pathogen of heart rot disease. The antibacterial activity was evaluated using well diffusion method where three concentrations for each extracts were prepared. The results revealed that Vernonia amygdalina chloroform and aqueous leaves extracts at 10000ppm shows greater inhibition zone than positive control. Vernonia amygdalina hexane leaves extracts however did not shows any inhibition zone at any concentrations. Meanwhile, the Vernonia amygdalina methanol leaves extract shows antibacterial activity against pathogen of heart rot disease but not as good as positive control. Phytochemical screening was also carried out to determine alkaloid, flavanoid, glycoside, phenol, saponin, steroid, and tannins as these compounds may attribute to the result. The screening revealed that both Vernonia amygdalina chloroform and aqueous leaves extracts contain alkaloid, saponin, and tannin. Result shows the potentials of Vernonia amygdalina as antibacterial agent in combating plant disease infections.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	5626/54495
Uncontrolled Keywords:	Antibacterial activtity, Heart rot disease, Inhibition zone, Phytochemical screening, Vernonia amygdalina
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Prof Dr Muhammad Nor Omar
Date Deposited:	02 Feb 2017 16:14
Last Modified:	02 Feb 2017 16:14
URI:	http://irep.iium.edu.my/id/eprint/54495

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