Kinetic study of acetic acid (vinegar) production from start fruit juice in a bioreactor

Abdul Karim, Mohamed Ismail and Jamal, Parveen and Kamaruddin, Noor Izzaida (2014) Kinetic study of acetic acid (vinegar) production from start fruit juice in a bioreactor. Journal of Pure and Applied Microbiology, 8 (Special Issue). pp. 853-857. ISSN 0973-7510

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Abstract

A two stage fermentation process for production of acetic acid using star fruit juice with Saccaromycescerevisiae and Acetomonasacetii was conducted in a 2 L stirred tank bioreactor. The effect of inoculum size ( 5%,10%,15%) and agitation speed ( 250 rpm, 300 rpm, 350 rpm) on ethanol and acetic acid production of the juice were evaluated. Kinetic parameters on specific growth rate, yield, doubling time (i.e. ?,Yp/s. td,) of the fermentation process were also recorded. The optimum condition obtained from this batch fermentation was at 250 rpm of agitation speed and 5% inoculums sizes with the maximum product yield (Yethanol/glucose) of 0.859 g/g and (Y Acetic acid/Ethanol) of 0.342 g/g. The maximum specific growth rate, ? was 0.097h-1 for Saccharomyces cerevisiae while 0.085h-1 for Acetobacteraceti using initial glucose concentration of 20%. Using Central Composite Design experiment, for optimization of acetic acid production, the R2 value of 0.8372 was achieved and the highest acetic acid production obtained was 2.76 %TA while highest ethanol obtained was 17.8% or equivalent to 140.64 g/L. The above findings can be further utilized in fermentation process to produce vinegar in large amount from our tropical star fruit, Averrhoacarambola juice.

Item Type:	Article (Journal)
Additional Information:	4684/53396
Uncontrolled Keywords:	Star fruit vinegar, bioreactor, agitation speed, inoculums size.
Subjects:	T Technology > TA Engineering (General). Civil engineering (General) > TA164 Bioengineering
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof. Dr. Mohamed Ismail Abdul Karim
Date Deposited:	14 Feb 2017 11:56
Last Modified:	29 Jan 2018 08:32
URI:	http://irep.iium.edu.my/id/eprint/53396

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